Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9875 | 5' | -52.5 | NC_002641.1 | + | 31589 | 0.66 | 0.984376 |
Target: 5'- -gGGAUGuGGGACUguuggacaugcauGCCUAUguaaaGGUCCa -3' miRNA: 3'- agCCUACuCCUUGG-------------CGGGUAa----CUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 143545 | 0.66 | 0.980593 |
Target: 5'- gCGGAgUGGGGGAaCGCCCcccaGUCCu -3' miRNA: 3'- aGCCU-ACUCCUUgGCGGGuaacUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 106888 | 0.66 | 0.979716 |
Target: 5'- aUCGGAUGuccaucuugaagcGGAuUCGUCCAuUUGAUCUa -3' miRNA: 3'- -AGCCUACu------------CCUuGGCGGGU-AACUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 124838 | 0.67 | 0.973284 |
Target: 5'- uUCGGAUGGGaAGCC-CCCA---AUCCc -3' miRNA: 3'- -AGCCUACUCcUUGGcGGGUaacUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 157635 | 0.67 | 0.973284 |
Target: 5'- uUCGGAUGGGaAGCC-CCCA---AUCCc -3' miRNA: 3'- -AGCCUACUCcUUGGcGGGUaacUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 118347 | 0.68 | 0.964164 |
Target: 5'- cCGuc--GGGAACCGCCaCAUUGAccggUCCg -3' miRNA: 3'- aGCcuacUCCUUGGCGG-GUAACU----AGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 5375 | 0.68 | 0.964164 |
Target: 5'- cCGuc--GGGAACCGCCaCAUUGAccggUCCg -3' miRNA: 3'- aGCcuacUCCUUGGCGG-GUAACU----AGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 154318 | 0.68 | 0.956991 |
Target: 5'- gUGGAUGcGGAGaCgGCCCGUccGAUUCg -3' miRNA: 3'- aGCCUACuCCUU-GgCGGGUAa-CUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 128154 | 0.68 | 0.956991 |
Target: 5'- gUGGAUGcGGAGaCgGCCCGUccGAUUCg -3' miRNA: 3'- aGCCUACuCCUU-GgCGGGUAa-CUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 125811 | 0.68 | 0.953061 |
Target: 5'- -aGGAUGcGGAacuACCGUCUuuacgGAUCCg -3' miRNA: 3'- agCCUACuCCU---UGGCGGGuaa--CUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 156661 | 0.68 | 0.953061 |
Target: 5'- -aGGAUGcGGAacuACCGUCUuuacgGAUCCg -3' miRNA: 3'- agCCUACuCCU---UGGCGGGuaa--CUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 147025 | 0.68 | 0.948897 |
Target: 5'- gCGGggGAGGGacacuauggaagGCCGCCaCAcgGGUCa -3' miRNA: 3'- aGCCuaCUCCU------------UGGCGG-GUaaCUAGg -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 135447 | 0.68 | 0.948897 |
Target: 5'- gCGGggGAGGGacacuauggaagGCCGCCaCAcgGGUCa -3' miRNA: 3'- aGCCuaCUCCU------------UGGCGG-GUaaCUAGg -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 105194 | 0.68 | 0.948467 |
Target: 5'- -aGGAUGAcaucucuGGAGCCGCCac-UGAUUa -3' miRNA: 3'- agCCUACU-------CCUUGGCGGguaACUAGg -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 127899 | 0.69 | 0.93985 |
Target: 5'- aCGGgcGuaGGGAACCGUUCAguaUUGAUUCg -3' miRNA: 3'- aGCCuaC--UCCUUGGCGGGU---AACUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 154573 | 0.69 | 0.93985 |
Target: 5'- aCGGgcGuaGGGAACCGUUCAguaUUGAUUCg -3' miRNA: 3'- aGCCuaC--UCCUUGGCGGGU---AACUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 73393 | 0.69 | 0.929831 |
Target: 5'- aUGGuagcaAUGGGGggUCGCCUAUaGGUUCg -3' miRNA: 3'- aGCC-----UACUCCuuGGCGGGUAaCUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 9796 | 0.7 | 0.900503 |
Target: 5'- cUCGGGaGGcGGAACCGCCCAaccagCCc -3' miRNA: 3'- -AGCCUaCU-CCUUGGCGGGUaacuaGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 59027 | 0.75 | 0.659777 |
Target: 5'- cUUGGAUcGGGAACgGCCacauGUUGAUCCu -3' miRNA: 3'- -AGCCUAcUCCUUGgCGGg---UAACUAGG- -5' |
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9875 | 5' | -52.5 | NC_002641.1 | + | 38640 | 1.12 | 0.00467 |
Target: 5'- cUCGGAUGAGGAACCGCCCAUUGAUCCc -3' miRNA: 3'- -AGCCUACUCCUUGGCGGGUAACUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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