Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9876 | 3' | -55.2 | NC_002641.1 | + | 99040 | 0.69 | 0.827889 |
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Target: 5'- gUGCGCGAUcgagacgAUgGACGCCGUCucgaUCGCg -3' miRNA: 3'- -AUGCGUUA-------UAgCUGCGGCAGcg--GGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 21145 | 0.69 | 0.820245 |
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Target: 5'- cACGUcuccgCGACGCCGUUuugGCUCGCc -3' miRNA: 3'- aUGCGuuauaGCUGCGGCAG---CGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 128344 | 0.69 | 0.802773 |
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Target: 5'- gGCGCGA-AUCGGacggGCCGUC-UCCGCa -3' miRNA: 3'- aUGCGUUaUAGCUg---CGGCAGcGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 154128 | 0.69 | 0.802773 |
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Target: 5'- gGCGCGA-AUCGGacggGCCGUC-UCCGCa -3' miRNA: 3'- aUGCGUUaUAGCUg---CGGCAGcGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 40219 | 0.7 | 0.775431 |
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Target: 5'- gUACGaAAUAUgGACGaCGUCGCUCGUu -3' miRNA: 3'- -AUGCgUUAUAgCUGCgGCAGCGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 69777 | 0.7 | 0.775431 |
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Target: 5'- -cCGUGAUG-CGACGUCGUCGgCgGCa -3' miRNA: 3'- auGCGUUAUaGCUGCGGCAGCgGgCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 67042 | 0.71 | 0.74694 |
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Target: 5'- gAUGCGAguaaGACGuaugacacagaaCCGUUGCCCGCg -3' miRNA: 3'- aUGCGUUauagCUGC------------GGCAGCGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 52216 | 0.75 | 0.506089 |
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Target: 5'- aUACGcCAGUGUCGG-GCCcggCGCCCGCc -3' miRNA: 3'- -AUGC-GUUAUAGCUgCGGca-GCGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 98032 | 0.76 | 0.431534 |
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Target: 5'- gUAUGCAA---UGGCGCCGUaacaaGCCCGCa -3' miRNA: 3'- -AUGCGUUauaGCUGCGGCAg----CGGGCG- -5' |
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| 9876 | 3' | -55.2 | NC_002641.1 | + | 60664 | 1.11 | 0.002875 |
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Target: 5'- gUACGCAAUAUCGACGCCGUCGCCCGCg -3' miRNA: 3'- -AUGCGUUAUAGCUGCGGCAGCGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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