Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9877 | 3' | -55.9 | NC_002641.1 | + | 67325 | 0.67 | 0.879273 |
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Target: 5'- ---aGGcACGCCuCCAUCU-GUUCGGAa -3' miRNA: 3'- ucaaCC-UGCGGcGGUAGGuCAAGCCU- -5' |
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| 9877 | 3' | -55.9 | NC_002641.1 | + | 138124 | 0.68 | 0.84127 |
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Target: 5'- ---gGGAgGCCGCCAUCagcaggAGUUUGGc -3' miRNA: 3'- ucaaCCUgCGGCGGUAGg-----UCAAGCCu -5' |
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| 9877 | 3' | -55.9 | NC_002641.1 | + | 5578 | 0.71 | 0.703092 |
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Target: 5'- cGGggGGACGCCGCCGcggaCCGGUcaauguggCGGu -3' miRNA: 3'- -UCaaCCUGCGGCGGUa---GGUCAa-------GCCu -5' |
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| 9877 | 3' | -55.9 | NC_002641.1 | + | 118144 | 0.71 | 0.703092 |
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Target: 5'- cGGggGGACGCCGCCGcggaCCGGUcaauguggCGGu -3' miRNA: 3'- -UCaaCCUGCGGCGGUa---GGUCAa-------GCCu -5' |
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| 9877 | 3' | -55.9 | NC_002641.1 | + | 59057 | 0.72 | 0.632166 |
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Target: 5'- ---cGGACGCCGCaaaGUCCAGgcUCGcGAa -3' miRNA: 3'- ucaaCCUGCGGCGg--UAGGUCa-AGC-CU- -5' |
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| 9877 | 3' | -55.9 | NC_002641.1 | + | 77946 | 1.09 | 0.00304 |
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Target: 5'- gAGUUGGACGCCGCCAUCCAGUUCGGAa -3' miRNA: 3'- -UCAACCUGCGGCGGUAGGUCAAGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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