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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9879 | 3' | -47.8 | NC_002641.1 | + | 103354 | 0.69 | 0.992679 |
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Target: 5'- gGGUGCCGGGAuau--CAUAUCCCgCGu -3' miRNA: 3'- -CUAUGGCCUUuuauuGUAUGGGGgGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 127156 | 0.7 | 0.991553 |
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Target: 5'- --cACCuGGggGAUGAUAUGCCCgugCCUAc -3' miRNA: 3'- cuaUGG-CCuuUUAUUGUAUGGG---GGGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 155317 | 0.7 | 0.991553 |
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Target: 5'- --cACCuGGggGAUGAUAUGCCCgugCCUAc -3' miRNA: 3'- cuaUGG-CCuuUUAUUGUAUGGG---GGGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 13603 | 0.7 | 0.990296 |
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Target: 5'- --cACgGGcgGGUAACAUAUCCCUCGu -3' miRNA: 3'- cuaUGgCCuuUUAUUGUAUGGGGGGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 151286 | 0.71 | 0.981675 |
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Target: 5'- cGGUACgagaggCGGggGAUGGCAgacACCaCCCCGg -3' miRNA: 3'- -CUAUG------GCCuuUUAUUGUa--UGG-GGGGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 131186 | 0.71 | 0.981675 |
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Target: 5'- cGGUACgagaggCGGggGAUGGCAgacACCaCCCCGg -3' miRNA: 3'- -CUAUG------GCCuuUUAUUGUa--UGG-GGGGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 153947 | 0.72 | 0.964939 |
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Target: 5'- --cACCGGggGGUAguuuccgcgcGCccGCCCCCCu -3' miRNA: 3'- cuaUGGCCuuUUAU----------UGuaUGGGGGGu -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 128526 | 0.72 | 0.964939 |
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Target: 5'- --cACCGGggGGUAguuuccgcgcGCccGCCCCCCu -3' miRNA: 3'- cuaUGGCCuuUUAU----------UGuaUGGGGGGu -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 114943 | 0.81 | 0.599449 |
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Target: 5'- cGGUGCa-GAAGAUAACAaGCCCCCCAg -3' miRNA: 3'- -CUAUGgcCUUUUAUUGUaUGGGGGGU- -5' |
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| 9879 | 3' | -47.8 | NC_002641.1 | + | 104000 | 1.09 | 0.017922 |
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Target: 5'- uGAUACCGGAAAAUAACAUACCCCCCAc -3' miRNA: 3'- -CUAUGGCCUUUUAUUGUAUGGGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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