Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9879 | 5' | -53.7 | NC_002641.1 | + | 38350 | 0.66 | 0.97136 |
Target: 5'- uGUAAGGAGCGCaUCUGCA---UCGGCa -3' miRNA: 3'- cCAUUUCUCGCG-GGAUGUcugGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 155651 | 0.66 | 0.968392 |
Target: 5'- uGGUAAgaggGGGGCGCCU---AGACCCc-- -3' miRNA: 3'- -CCAUU----UCUCGCGGGaugUCUGGGcug -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 126821 | 0.66 | 0.968392 |
Target: 5'- uGGUAAgaggGGGGCGCCU---AGACCCc-- -3' miRNA: 3'- -CCAUU----UCUCGCGGGaugUCUGGGcug -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 126574 | 0.66 | 0.961814 |
Target: 5'- --aAGGGAGUGuUCCcGCcGACCCGGCu -3' miRNA: 3'- ccaUUUCUCGC-GGGaUGuCUGGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 52208 | 0.66 | 0.961814 |
Target: 5'- aGGUAcuGAuaCGCCaguguCGGGCCCGGCg -3' miRNA: 3'- -CCAUuuCUc-GCGGgau--GUCUGGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 155898 | 0.66 | 0.961814 |
Target: 5'- --aAGGGAGUGuUCCcGCcGACCCGGCu -3' miRNA: 3'- ccaUUUCUCGC-GGGaUGuCUGGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 129860 | 0.66 | 0.958192 |
Target: 5'- cGGUGGGGucGGCGgUC-ACcGACCCGGCc -3' miRNA: 3'- -CCAUUUC--UCGCgGGaUGuCUGGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 152613 | 0.66 | 0.958192 |
Target: 5'- cGGUGGGGucGGCGgUC-ACcGACCCGGCc -3' miRNA: 3'- -CCAUUUC--UCGCgGGaUGuCUGGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 146969 | 0.67 | 0.941379 |
Target: 5'- cGGUugccGGGGAGCGgUCUugccgagcagGCAGACCCuGCa -3' miRNA: 3'- -CCA----UUUCUCGCgGGA----------UGUCUGGGcUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 135504 | 0.67 | 0.941379 |
Target: 5'- cGGUugccGGGGAGCGgUCUugccgagcagGCAGACCCuGCa -3' miRNA: 3'- -CCA----UUUCUCGCgGGA----------UGUCUGGGcUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 52932 | 0.69 | 0.896148 |
Target: 5'- aGUGGAuacaauGGC-CCCUGCAGcCCCGACu -3' miRNA: 3'- cCAUUUc-----UCGcGGGAUGUCuGGGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 11888 | 0.71 | 0.792132 |
Target: 5'- cGGggGGGAGC-CCCU-CAGGCCgcCGACg -3' miRNA: 3'- -CCauUUCUCGcGGGAuGUCUGG--GCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 65069 | 0.73 | 0.684192 |
Target: 5'- --cAGAGGGUGCCCaACAGAUgCGAUa -3' miRNA: 3'- ccaUUUCUCGCGGGaUGUCUGgGCUG- -5' |
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9879 | 5' | -53.7 | NC_002641.1 | + | 103966 | 1.13 | 0.002712 |
Target: 5'- cGGUAAAGAGCGCCCUACAGACCCGACa -3' miRNA: 3'- -CCAUUUCUCGCGGGAUGUCUGGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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