Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9882 | 3' | -47.9 | NC_002641.1 | + | 28988 | 0.66 | 0.999755 |
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Target: 5'- aGGCGUUCgGCAUUgGAGGgCAGUGGa -3' miRNA: 3'- cCUGCAAG-CGUGGaUUUCgGUUAUCa -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 5921 | 0.66 | 0.999755 |
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Target: 5'- -aGCGUUCGCACUgcGAaCCAAUAa- -3' miRNA: 3'- ccUGCAAGCGUGGauUUcGGUUAUca -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 117801 | 0.66 | 0.999755 |
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Target: 5'- -aGCGUUCGCACUgcGAaCCAAUAa- -3' miRNA: 3'- ccUGCAAGCGUGGauUUcGGUUAUca -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 155548 | 0.66 | 0.999606 |
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Target: 5'- uGGGCGgaUGCuuGCCUAGuggcGGCgCAAUAGa -3' miRNA: 3'- -CCUGCaaGCG--UGGAUU----UCG-GUUAUCa -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 126924 | 0.66 | 0.999606 |
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Target: 5'- uGGGCGgaUGCuuGCCUAGuggcGGCgCAAUAGa -3' miRNA: 3'- -CCUGCaaGCG--UGGAUU----UCG-GUUAUCa -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 89749 | 0.67 | 0.999238 |
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Target: 5'- aGAUGga-GCGCCaAucGCCGAUAGUu -3' miRNA: 3'- cCUGCaagCGUGGaUuuCGGUUAUCA- -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 57198 | 0.68 | 0.996572 |
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Target: 5'- cGACGUaaUCGCACaac--GCCGAUAGc -3' miRNA: 3'- cCUGCA--AGCGUGgauuuCGGUUAUCa -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 118022 | 1.12 | 0.012179 |
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Target: 5'- cGGACGUUCGCACCUAAAGCCAAUAGUa -3' miRNA: 3'- -CCUGCAAGCGUGGAUUUCGGUUAUCA- -5' |
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| 9882 | 3' | -47.9 | NC_002641.1 | + | 5701 | 1.12 | 0.012179 |
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Target: 5'- cGGACGUUCGCACCUAAAGCCAAUAGUa -3' miRNA: 3'- -CCUGCAAGCGUGGAUUUCGGUUAUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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