Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9882 | 5' | -46 | NC_002641.1 | + | 117020 | 0.66 | 0.999982 |
Target: 5'- ----aAUUGGagUCGCGGUcGUG-GCGCg -3' miRNA: 3'- uauaaUAACCa-AGCGUCA-CGCuUGCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 102419 | 0.67 | 0.999815 |
Target: 5'- ------gUGGUUCuaGCAGUcgacgGCGAGCGUu -3' miRNA: 3'- uauaauaACCAAG--CGUCA-----CGCUUGCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 84472 | 0.69 | 0.999056 |
Target: 5'- ------gUGGUgUGUAGUGCGGuCGCg -3' miRNA: 3'- uauaauaACCAaGCGUCACGCUuGCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 136119 | 0.7 | 0.997924 |
Target: 5'- ------gUGGUUCGCAGaugGCGGuccCGCc -3' miRNA: 3'- uauaauaACCAAGCGUCa--CGCUu--GCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 146354 | 0.7 | 0.997924 |
Target: 5'- ------gUGGUUCGCAGaugGCGGuccCGCc -3' miRNA: 3'- uauaauaACCAAGCGUCa--CGCUu--GCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 5888 | 0.71 | 0.99509 |
Target: 5'- uAUAcUAUUGGcUUuaGGUGCGAACGUc -3' miRNA: 3'- -UAUaAUAACCaAGcgUCACGCUUGCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 117834 | 0.71 | 0.99509 |
Target: 5'- uAUAcUAUUGGcUUuaGGUGCGAACGUc -3' miRNA: 3'- -UAUaAUAACCaAGcgUCACGCUUGCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 117987 | 1.11 | 0.019774 |
Target: 5'- uAUAUUAUUGGUUCGCAGUGCGAACGCu -3' miRNA: 3'- -UAUAAUAACCAAGCGUCACGCUUGCG- -5' |
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9882 | 5' | -46 | NC_002641.1 | + | 5736 | 1.11 | 0.019774 |
Target: 5'- uAUAUUAUUGGUUCGCAGUGCGAACGCu -3' miRNA: 3'- -UAUAAUAACCAAGCGUCACGCUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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