Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9883 | 5' | -47.9 | NC_002641.1 | + | 138970 | 0.67 | 0.99864 |
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Target: 5'- cCGCUCGUUACGAgucguuGGACAUAUcuguaauacaUUUCu -3' miRNA: 3'- -GUGGGCAAUGCUac----CCUGUGUA----------AAAG- -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 104393 | 0.68 | 0.997176 |
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Target: 5'- uGCCUG-UACucUGGGuCGCGUUUUCu -3' miRNA: 3'- gUGGGCaAUGcuACCCuGUGUAAAAG- -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 154201 | 0.69 | 0.996054 |
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Target: 5'- gGCCCGUaAUG-UGGGugGCAcUUUUUg -3' miRNA: 3'- gUGGGCAaUGCuACCCugUGU-AAAAG- -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 128271 | 0.69 | 0.996054 |
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Target: 5'- gGCCCGUaAUG-UGGGugGCAcUUUUUg -3' miRNA: 3'- gUGGGCAaUGCuACCCugUGU-AAAAG- -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 63032 | 0.69 | 0.995368 |
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Target: 5'- gUACCCgGUUACGAauucUGGGGCGUAUUg-- -3' miRNA: 3'- -GUGGG-CAAUGCU----ACCCUGUGUAAaag -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 4834 | 0.75 | 0.877721 |
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Target: 5'- -uCCUGUUACGAUGGGGCGguCAUUa-- -3' miRNA: 3'- guGGGCAAUGCUACCCUGU--GUAAaag -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 118888 | 0.75 | 0.877721 |
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Target: 5'- -uCCUGUUACGAUGGGGCGguCAUUa-- -3' miRNA: 3'- guGGGCAAUGCUACCCUGU--GUAAaag -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 119039 | 1.11 | 0.014214 |
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Target: 5'- aCACCCGUUACGAUGGGACACAUUUUCu -3' miRNA: 3'- -GUGGGCAAUGCUACCCUGUGUAAAAG- -5' |
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| 9883 | 5' | -47.9 | NC_002641.1 | + | 4684 | 1.11 | 0.014214 |
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Target: 5'- aCACCCGUUACGAUGGGACACAUUUUCu -3' miRNA: 3'- -GUGGGCAAUGCUACCCUGUGUAAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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