Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9884 | 3' | -52.7 | NC_002641.1 | + | 34169 | 0.66 | 0.985525 |
Target: 5'- ---uAUggCUGGAgaAGAGACCCaGCGGg -3' miRNA: 3'- cacuUAaaGGCCU--UCUCUGGG-CGCCg -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 146470 | 0.66 | 0.983713 |
Target: 5'- -gGAGUUUCCGGccaAGcCCCGCGa- -3' miRNA: 3'- caCUUAAAGGCCuucUCuGGGCGCcg -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 39961 | 0.66 | 0.983713 |
Target: 5'- -aGAAUUUCCGaGAGAuauGCCCGCcauGCa -3' miRNA: 3'- caCUUAAAGGCcUUCUc--UGGGCGc--CG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 136002 | 0.66 | 0.983713 |
Target: 5'- -gGAGUUUCCGGccaAGcCCCGCGa- -3' miRNA: 3'- caCUUAAAGGCCuucUCuGGGCGCcg -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 108702 | 0.66 | 0.977242 |
Target: 5'- uUGGAUUggCUGGAAGGGugcuaaCCGCauGGCa -3' miRNA: 3'- cACUUAAa-GGCCUUCUCug----GGCG--CCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 119718 | 0.66 | 0.974714 |
Target: 5'- -aGGAc--CCGGAAGAGACgCGCcaGCa -3' miRNA: 3'- caCUUaaaGGCCUUCUCUGgGCGc-CG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 123737 | 0.66 | 0.974714 |
Target: 5'- -aGAAUUUucccauucCCGGAc-AGACCCGCagccGGCa -3' miRNA: 3'- caCUUAAA--------GGCCUucUCUGGGCG----CCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 4004 | 0.66 | 0.974714 |
Target: 5'- -aGGAc--CCGGAAGAGACgCGCcaGCa -3' miRNA: 3'- caCUUaaaGGCCUUCUCUGgGCGc-CG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 93803 | 0.67 | 0.967193 |
Target: 5'- -aGAGUUUCCGaGGguuauuguggauacuAGuguGGCCCcGCGGCc -3' miRNA: 3'- caCUUAAAGGC-CU---------------UCu--CUGGG-CGCCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 117746 | 0.67 | 0.962545 |
Target: 5'- aUGug-UUagGGGAGAGACCCGUgaucGGCa -3' miRNA: 3'- cACuuaAAggCCUUCUCUGGGCG----CCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 11895 | 0.68 | 0.95514 |
Target: 5'- aUGAAg--CCGGggGGGAgCCCcucaGGCc -3' miRNA: 3'- cACUUaaaGGCCuuCUCU-GGGcg--CCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 37606 | 0.68 | 0.951089 |
Target: 5'- cUGGAUUcaaaagCGGggGGGAUCCGCGuGUc -3' miRNA: 3'- cACUUAAag----GCCuuCUCUGGGCGC-CG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 158743 | 0.7 | 0.876355 |
Target: 5'- aGUGAAUagaauuuucccaUUCCGcGGAcAGACCCGCagccGGCa -3' miRNA: 3'- -CACUUA------------AAGGC-CUUcUCUGGGCG----CCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 119936 | 1.13 | 0.003525 |
Target: 5'- cGUGAAUUUCCGGAAGAGACCCGCGGCa -3' miRNA: 3'- -CACUUAAAGGCCUUCUCUGGGCGCCG- -5' |
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9884 | 3' | -52.7 | NC_002641.1 | + | 3787 | 1.13 | 0.003525 |
Target: 5'- cGUGAAUUUCCGGAAGAGACCCGCGGCa -3' miRNA: 3'- -CACUUAAAGGCCUUCUCUGGGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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