Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9885 | 3' | -54.2 | NC_002641.1 | + | 137894 | 0.66 | 0.958366 |
Target: 5'- cGgGACGCCGUCAUGAcGCUCGauuaauGUAg -3' miRNA: 3'- -CgUUGUGGCGGUGCUaCGAGUgu----CGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 5573 | 0.66 | 0.954509 |
Target: 5'- -gGACGCCGCCGCGGaccGgUCAauguGGCGg -3' miRNA: 3'- cgUUGUGGCGGUGCUa--CgAGUg---UCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 118149 | 0.66 | 0.954509 |
Target: 5'- -gGACGCCGCCGCGGaccGgUCAauguGGCGg -3' miRNA: 3'- cgUUGUGGCGGUGCUa--CgAGUg---UCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 47252 | 0.66 | 0.954509 |
Target: 5'- ---uCGCCGCCACagGGUGCcuaUC-CAGCAu -3' miRNA: 3'- cguuGUGGCGGUG--CUACG---AGuGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 3902 | 0.66 | 0.954509 |
Target: 5'- cCAGCucguCCGCCGCGAaagUGCcggUCcCGGCGa -3' miRNA: 3'- cGUUGu---GGCGGUGCU---ACG---AGuGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 119821 | 0.66 | 0.954509 |
Target: 5'- cCAGCucguCCGCCGCGAaagUGCcggUCcCGGCGa -3' miRNA: 3'- cGUUGu---GGCGGUGCU---ACG---AGuGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 68391 | 0.66 | 0.946088 |
Target: 5'- -gAGCGCCGCUcC-AUGCUCACAuaccuGCAu -3' miRNA: 3'- cgUUGUGGCGGuGcUACGAGUGU-----CGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 157427 | 0.66 | 0.936206 |
Target: 5'- cCAGcCACCagGCCGCGAcGCUgauacacCGCAGCAu -3' miRNA: 3'- cGUU-GUGG--CGGUGCUaCGA-------GUGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 125046 | 0.66 | 0.936206 |
Target: 5'- cCAGcCACCagGCCGCGAcGCUgauacacCGCAGCAu -3' miRNA: 3'- cGUU-GUGG--CGGUGCUaCGA-------GUGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 125279 | 0.67 | 0.925788 |
Target: 5'- cGCAGCucuGCCGCCguaggacucggcgGCGGcccuuccuUGC-CGCAGCAg -3' miRNA: 3'- -CGUUG---UGGCGG-------------UGCU--------ACGaGUGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 157193 | 0.67 | 0.920778 |
Target: 5'- cGCAGCucuGCCGCCguaggacucgGCGGggcccuuccuUGC-CGCAGCAg -3' miRNA: 3'- -CGUUG---UGGCGG----------UGCU----------ACGaGUGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 64076 | 0.68 | 0.889345 |
Target: 5'- gGCGACACCGacuCGGUGUUCAUuaaauucacgGGCGu -3' miRNA: 3'- -CGUUGUGGCgguGCUACGAGUG----------UCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 37842 | 0.68 | 0.881631 |
Target: 5'- cGCAAUGCCGUgACGGauaugggaaacacUGCUCAgAGUu -3' miRNA: 3'- -CGUUGUGGCGgUGCU-------------ACGAGUgUCGu -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 38108 | 0.69 | 0.852099 |
Target: 5'- cGCAuuGCCGCCA-GAUGaUCAgAGCAu -3' miRNA: 3'- -CGUugUGGCGGUgCUACgAGUgUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 98173 | 0.71 | 0.7338 |
Target: 5'- aGCcAUACCGUCGCGGUGCacauUgGCGGCu -3' miRNA: 3'- -CGuUGUGGCGGUGCUACG----AgUGUCGu -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 141314 | 0.73 | 0.652036 |
Target: 5'- cGC-ACGCCGCU-CGuUGUUCACGGCAc -3' miRNA: 3'- -CGuUGUGGCGGuGCuACGAGUGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 153735 | 0.75 | 0.53863 |
Target: 5'- aGCGACACCG-CGCG-UGC-CGCGGCGg -3' miRNA: 3'- -CGUUGUGGCgGUGCuACGaGUGUCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 157572 | 0.77 | 0.414731 |
Target: 5'- cCAGCGCCGCgCACGGUGCUCcucGCuucuGGCAg -3' miRNA: 3'- cGUUGUGGCG-GUGCUACGAG---UG----UCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 124900 | 0.77 | 0.414731 |
Target: 5'- cCAGCGCCGCgCACGGUGCUCcucGCuucuGGCAg -3' miRNA: 3'- cGUUGUGGCG-GUGCUACGAG---UG----UCGU- -5' |
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9885 | 3' | -54.2 | NC_002641.1 | + | 120105 | 1.11 | 0.003033 |
Target: 5'- cGCAACACCGCCACGAUGCUCACAGCAu -3' miRNA: 3'- -CGUUGUGGCGGUGCUACGAGUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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