Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9885 | 5' | -58.5 | NC_002641.1 | + | 66071 | 0.66 | 0.820815 |
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Target: 5'- cACCGGCGUcuuucacaaacgcagAuaauGCGGUCggaCGGGGCGG-GAa -3' miRNA: 3'- -UGGCCGUA---------------U----CGUUAG---GCCCCGCCaCU- -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 108724 | 0.68 | 0.706078 |
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Target: 5'- aACC-GCAUGGCAugcCCGcGGGCGGa-- -3' miRNA: 3'- -UGGcCGUAUCGUua-GGC-CCCGCCacu -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 153431 | 0.68 | 0.696197 |
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Target: 5'- cGCCGGCAgccGC--UCCGGGGCcuacGGgGAg -3' miRNA: 3'- -UGGCCGUau-CGuuAGGCCCCG----CCaCU- -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 129041 | 0.68 | 0.696197 |
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Target: 5'- cGCCGGCAgccGC--UCCGGGGCcuacGGgGAg -3' miRNA: 3'- -UGGCCGUau-CGuuAGGCCCCG----CCaCU- -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 38257 | 0.7 | 0.553111 |
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Target: 5'- uCUGGUGgauGCAgaugcgccgccuauAUCCGGGGUGGUGGc -3' miRNA: 3'- uGGCCGUau-CGU--------------UAGGCCCCGCCACU- -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 52299 | 0.71 | 0.526716 |
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Target: 5'- aGCCGGUAUcggaGGUAA-CCGGGuGCGGUa- -3' miRNA: 3'- -UGGCCGUA----UCGUUaGGCCC-CGCCAcu -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 120068 | 1.08 | 0.002021 |
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Target: 5'- cACCGGCAUAGCAAUCCGGGGCGGUGAu -3' miRNA: 3'- -UGGCCGUAUCGUUAGGCCCCGCCACU- -5' |
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| 9885 | 5' | -58.5 | NC_002641.1 | + | 3655 | 1.08 | 0.002021 |
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Target: 5'- cACCGGCAUAGCAAUCCGGGGCGGUGAu -3' miRNA: 3'- -UGGCCGUAUCGUUAGGCCCCGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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