Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9889 | 5' | -53.6 | NC_002641.1 | + | 26494 | 0.66 | 0.957074 |
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Target: 5'- cGGCAGCcgGCGCuuucuaucUCACCGuAGAAu-- -3' miRNA: 3'- uCCGUCGa-UGCG--------AGUGGCcUCUUuua -5' |
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| 9889 | 5' | -53.6 | NC_002641.1 | + | 1938 | 0.66 | 0.944343 |
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Target: 5'- -aGCAGCUACGUUguCCGcGGGAu--- -3' miRNA: 3'- ucCGUCGAUGCGAguGGC-CUCUuuua -5' |
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| 9889 | 5' | -53.6 | NC_002641.1 | + | 121784 | 0.66 | 0.944343 |
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Target: 5'- -aGCAGCUACGUUguCCGcGGGAu--- -3' miRNA: 3'- ucCGUCGAUGCGAguGGC-CUCUuuua -5' |
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| 9889 | 5' | -53.6 | NC_002641.1 | + | 37566 | 0.67 | 0.918111 |
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Target: 5'- cGGGCAGCUGgGCcaauaCACuCGGGuGAAGAc -3' miRNA: 3'- -UCCGUCGAUgCGa----GUG-GCCU-CUUUUa -5' |
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| 9889 | 5' | -53.6 | NC_002641.1 | + | 64704 | 0.69 | 0.863169 |
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Target: 5'- aGGGCGGCUcaGCUCGgCGGAcaugGAAAAg -3' miRNA: 3'- -UCCGUCGAugCGAGUgGCCU----CUUUUa -5' |
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| 9889 | 5' | -53.6 | NC_002641.1 | + | 121934 | 1.06 | 0.006018 |
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Target: 5'- cAGGCAGCUACGCUCACCGGAGAAAAUa -3' miRNA: 3'- -UCCGUCGAUGCGAGUGGCCUCUUUUA- -5' |
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| 9889 | 5' | -53.6 | NC_002641.1 | + | 1789 | 1.06 | 0.006018 |
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Target: 5'- cAGGCAGCUACGCUCACCGGAGAAAAUa -3' miRNA: 3'- -UCCGUCGAUGCGAGUGGCCUCUUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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