Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9891 | 3' | -50.9 | NC_002641.1 | + | 27105 | 0.66 | 0.994991 |
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Target: 5'- aCCacgCAGCCCAaucaCGUAUUc--GCUGCa -3' miRNA: 3'- -GGa--GUCGGGUac--GCAUAGaaaCGACG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 48295 | 0.66 | 0.994991 |
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Target: 5'- ---aAGCCCAUGCGUAaaaUUUGCc-- -3' miRNA: 3'- ggagUCGGGUACGCAUag-AAACGacg -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 152857 | 0.66 | 0.994991 |
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Target: 5'- uCCaggCAGCaCCccGCGUGUCggcauccGCUGUg -3' miRNA: 3'- -GGa--GUCG-GGuaCGCAUAGaaa----CGACG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 129615 | 0.66 | 0.994991 |
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Target: 5'- uCCaggCAGCaCCccGCGUGUCggcauccGCUGUg -3' miRNA: 3'- -GGa--GUCG-GGuaCGCAUAGaaa----CGACG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 6051 | 0.66 | 0.992232 |
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Target: 5'- gCCgCAGCCCGgcgGUGUcUCUUucagUGgaGCg -3' miRNA: 3'- -GGaGUCGGGUa--CGCAuAGAA----ACgaCG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 117672 | 0.66 | 0.992232 |
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Target: 5'- gCCgCAGCCCGgcgGUGUcUCUUucagUGgaGCg -3' miRNA: 3'- -GGaGUCGGGUa--CGCAuAGAA----ACgaCG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 12180 | 0.69 | 0.964544 |
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Target: 5'- aCUgGGCUCG-GCGUGgggugUUGCUGCu -3' miRNA: 3'- gGAgUCGGGUaCGCAUaga--AACGACG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 9349 | 0.7 | 0.93473 |
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Target: 5'- -aUCGGCCCGUGUGgaUAUCUaUGaaGCg -3' miRNA: 3'- ggAGUCGGGUACGC--AUAGAaACgaCG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 122322 | 1.15 | 0.003919 |
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Target: 5'- cCCUCAGCCCAUGCGUAUCUUUGCUGCu -3' miRNA: 3'- -GGAGUCGGGUACGCAUAGAAACGACG- -5' |
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| 9891 | 3' | -50.9 | NC_002641.1 | + | 1400 | 1.15 | 0.003919 |
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Target: 5'- cCCUCAGCCCAUGCGUAUCUUUGCUGCu -3' miRNA: 3'- -GGAGUCGGGUACGCAUAGAAACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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