Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9896 | 3' | -56.6 | NC_002641.1 | + | 154035 | 0.66 | 0.889543 |
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Target: 5'- -cGAgacUGUCCGUGgUUAUCUgGGCGg -3' miRNA: 3'- uaCUau-ACGGGCACgGAUGGAgCCGC- -5' |
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| 9896 | 3' | -56.6 | NC_002641.1 | + | 110407 | 0.66 | 0.889543 |
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Target: 5'- -aGAaaUAUcGCCCGUGCacaUACaUCGGCa -3' miRNA: 3'- uaCU--AUA-CGGGCACGg--AUGgAGCCGc -5' |
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| 9896 | 3' | -56.6 | NC_002641.1 | + | 128438 | 0.66 | 0.889543 |
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Target: 5'- -cGAgacUGUCCGUGgUUAUCUgGGCGg -3' miRNA: 3'- uaCUau-ACGGGCACgGAUGGAgCCGC- -5' |
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| 9896 | 3' | -56.6 | NC_002641.1 | + | 98311 | 0.7 | 0.645547 |
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Target: 5'- ------aGCCCGUGCCUG-CUUGGCu -3' miRNA: 3'- uacuauaCGGGCACGGAUgGAGCCGc -5' |
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| 9896 | 3' | -56.6 | NC_002641.1 | + | 127147 | 1.08 | 0.002873 |
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Target: 5'- gAUGAUAUGCCCGUGCCUACCUCGGCGg -3' miRNA: 3'- -UACUAUACGGGCACGGAUGGAGCCGC- -5' |
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| 9896 | 3' | -56.6 | NC_002641.1 | + | 155326 | 1.08 | 0.002873 |
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Target: 5'- gAUGAUAUGCCCGUGCCUACCUCGGCGg -3' miRNA: 3'- -UACUAUACGGGCACGGAUGGAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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