Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9896 | 5' | -58.1 | NC_002641.1 | + | 157249 | 0.66 | 0.816583 |
Target: 5'- aGgGCCaaGCCGCUuGGCcgcAGCGcuggCCGCg -3' miRNA: 3'- aCgCGG--CGGUGAuCCGu--UCGUa---GGCG- -5' |
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9896 | 5' | -58.1 | NC_002641.1 | + | 157269 | 0.68 | 0.684093 |
Target: 5'- cGUGCCGCCGCUcgcuaacGGCGcGCAggaGCg -3' miRNA: 3'- aCGCGGCGGUGAu------CCGUuCGUaggCG- -5' |
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9896 | 5' | -58.1 | NC_002641.1 | + | 157385 | 0.68 | 0.684093 |
Target: 5'- aGCGCCGUUGCca-GCGAGCcgCCGa -3' miRNA: 3'- aCGCGGCGGUGaucCGUUCGuaGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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