Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9898 | 3' | -63.1 | NC_002641.1 | + | 74648 | 0.66 | 0.649773 |
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Target: 5'- -cUGGCCC-GGACGCauagaucggAGGgGGCCa -3' miRNA: 3'- gcACCGGGcCCUGCGgga------UCCgCUGG- -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 104313 | 0.66 | 0.640153 |
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Target: 5'- uGUGGCcacccaCCGGGAaaaUGCCCagaGGGUcGCCg -3' miRNA: 3'- gCACCG------GGCCCU---GCGGGa--UCCGcUGG- -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 95671 | 0.67 | 0.582543 |
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Target: 5'- --gGGUCUGGGGgGCCUUcuuuGGCGAgCa -3' miRNA: 3'- gcaCCGGGCCCUgCGGGAu---CCGCUgG- -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 154026 | 0.68 | 0.534404 |
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Target: 5'- cCGUGGUUaucugggCGGGGuCGCCUaGGGCGuCCc -3' miRNA: 3'- -GCACCGG-------GCCCU-GCGGGaUCCGCuGG- -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 128447 | 0.68 | 0.534404 |
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Target: 5'- cCGUGGUUaucugggCGGGGuCGCCUaGGGCGuCCc -3' miRNA: 3'- -GCACCGG-------GCCCU-GCGGGaUCCGCuGG- -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 43200 | 0.7 | 0.38813 |
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Target: 5'- aGUGGCCauGGGCGCgCUGGuaGACg -3' miRNA: 3'- gCACCGGgcCCUGCGgGAUCcgCUGg -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 128649 | 1.1 | 0.000727 |
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Target: 5'- cCGUGGCCCGGGACGCCCUAGGCGACCc -3' miRNA: 3'- -GCACCGGGCCCUGCGGGAUCCGCUGG- -5' |
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| 9898 | 3' | -63.1 | NC_002641.1 | + | 153823 | 1.1 | 0.000727 |
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Target: 5'- cCGUGGCCCGGGACGCCCUAGGCGACCc -3' miRNA: 3'- -GCACCGGGCCCUGCGGGAUCCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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