Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
99 | 5' | -59 | AC_000006.1 | + | 9192 | 0.66 | 0.380523 |
Target: 5'- gCCGucgucGucGGGccccgccaccACCACCGCCcCCAGAg -3' miRNA: 3'- aGGCu----CuuCCC----------UGGUGGCGGaGGUCU- -5' |
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99 | 5' | -59 | AC_000006.1 | + | 4845 | 0.67 | 0.354393 |
Target: 5'- gCCGAGGAGGcGGuCC-CCGCCcagCgAGAg -3' miRNA: 3'- aGGCUCUUCC-CU-GGuGGCGGa--GgUCU- -5' |
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99 | 5' | -59 | AC_000006.1 | + | 25678 | 0.69 | 0.269711 |
Target: 5'- cCUGGGcguuGAGGuGACCGCCGCCugcUCCAa- -3' miRNA: 3'- aGGCUC----UUCC-CUGGUGGCGG---AGGUcu -5' |
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99 | 5' | -59 | AC_000006.1 | + | 4903 | 0.69 | 0.256187 |
Target: 5'- gCUGGGcGGGGACCGCCuCCUCgGcGAg -3' miRNA: 3'- aGGCUCuUCCCUGGUGGcGGAGgU-CU- -5' |
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99 | 5' | -59 | AC_000006.1 | + | 8698 | 0.69 | 0.249636 |
Target: 5'- -gCGAGAAGGcGCCcagGCCGCUcucguUCCAGAc -3' miRNA: 3'- agGCUCUUCCcUGG---UGGCGG-----AGGUCU- -5' |
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99 | 5' | -59 | AC_000006.1 | + | 17615 | 0.7 | 0.230812 |
Target: 5'- aCCG-GAGGGGGCUgcacaaccggaGCUGCCUUCGGu -3' miRNA: 3'- aGGCuCUUCCCUGG-----------UGGCGGAGGUCu -5' |
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99 | 5' | -59 | AC_000006.1 | + | 15964 | 0.72 | 0.162192 |
Target: 5'- aCCGAGGAccgcucgccGGGcgugaacACCACCGCCgugCCGGGc -3' miRNA: 3'- aGGCUCUU---------CCC-------UGGUGGCGGa--GGUCU- -5' |
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99 | 5' | -59 | AC_000006.1 | + | 20939 | 0.72 | 0.153916 |
Target: 5'- aCCGuGcAGGGGCCcgacucGCCGCCUgCGGAc -3' miRNA: 3'- aGGCuCuUCCCUGG------UGGCGGAgGUCU- -5' |
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99 | 5' | -59 | AC_000006.1 | + | 11982 | 1.09 | 0.000245 |
Target: 5'- gUCCGAGAAGGGACCACCGCCUCCAGAa -3' miRNA: 3'- -AGGCUCUUCCCUGGUGGCGGAGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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