Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9901 | 5' | -61.5 | NC_002641.1 | + | 71454 | 0.66 | 0.703005 |
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Target: 5'- cGUCGCuauaaacgGGCAauUCUucGCUGCCGGUGaGGg -3' miRNA: 3'- -UAGUG--------CCGU--GGGuuCGGCGGCCAC-CC- -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 146859 | 0.66 | 0.664003 |
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Target: 5'- --gACGGCAgCCAAuCC-CCGGUGGu -3' miRNA: 3'- uagUGCCGUgGGUUcGGcGGCCACCc -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 135613 | 0.66 | 0.664003 |
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Target: 5'- --gACGGCAgCCAAuCC-CCGGUGGu -3' miRNA: 3'- uagUGCCGUgGGUUcGGcGGCCACCc -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 127493 | 0.67 | 0.634473 |
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Target: 5'- -aCGgGGcCACCCAuaAGCCGCauCGG-GGGu -3' miRNA: 3'- uaGUgCC-GUGGGU--UCGGCG--GCCaCCC- -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 154980 | 0.67 | 0.634473 |
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Target: 5'- -aCGgGGcCACCCAuaAGCCGCauCGG-GGGu -3' miRNA: 3'- uaGUgCC-GUGGGU--UCGGCG--GCCaCCC- -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 146630 | 0.67 | 0.614765 |
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Target: 5'- --gGCGGCGa-CAGGaCCGCCugGGUGGGc -3' miRNA: 3'- uagUGCCGUggGUUC-GGCGG--CCACCC- -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 135842 | 0.67 | 0.614765 |
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Target: 5'- --gGCGGCGa-CAGGaCCGCCugGGUGGGc -3' miRNA: 3'- uagUGCCGUggGUUC-GGCGG--CCACCC- -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 36195 | 0.69 | 0.507617 |
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Target: 5'- --uGCGGCAUCCAGGCUuugaagaGCCGG-GGu -3' miRNA: 3'- uagUGCCGUGGGUUCGG-------CGGCCaCCc -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 132385 | 1.08 | 0.001056 |
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Target: 5'- cAUCACGGCACCCAAGCCGCCGGUGGGg -3' miRNA: 3'- -UAGUGCCGUGGGUUCGGCGGCCACCC- -5' |
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| 9901 | 5' | -61.5 | NC_002641.1 | + | 150087 | 1.08 | 0.001056 |
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Target: 5'- cAUCACGGCACCCAAGCCGCCGGUGGGg -3' miRNA: 3'- -UAGUGCCGUGGGUUCGGCGGCCACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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