Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9902 | 5' | -56.6 | NC_002641.1 | + | 31616 | 0.66 | 0.868332 |
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Target: 5'- aCACCUggacGUGuaugGUGGGAGuugGGGAUGUGg -3' miRNA: 3'- -GUGGG----UACua--CGCCCUC---UCCUACGCa -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 149038 | 0.66 | 0.857744 |
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Target: 5'- cUAUCCAggaugggacggggGAUG-GGGAGAGGAgGCGg -3' miRNA: 3'- -GUGGGUa------------CUACgCCCUCUCCUaCGCa -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 133435 | 0.66 | 0.857744 |
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Target: 5'- cUAUCCAggaugggacggggGAUG-GGGAGAGGAgGCGg -3' miRNA: 3'- -GUGGGUa------------CUACgCCCUCUCCUaCGCa -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 125601 | 0.68 | 0.802773 |
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Target: 5'- aCACCgGgaccgGAUgGCGGGGGGGGGguUGUGg -3' miRNA: 3'- -GUGGgUa----CUA-CGCCCUCUCCU--ACGCa -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 156871 | 0.68 | 0.802773 |
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Target: 5'- aCACCgGgaccgGAUgGCGGGGGGGGGguUGUGg -3' miRNA: 3'- -GUGGgUa----CUA-CGCCCUCUCCU--ACGCa -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 146606 | 0.69 | 0.74694 |
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Target: 5'- gGCCaCAUGugcGUGGGGGGGGAuuuccguuUGCGUc -3' miRNA: 3'- gUGG-GUACua-CGCCCUCUCCU--------ACGCA- -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 135866 | 0.69 | 0.74694 |
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Target: 5'- gGCCaCAUGugcGUGGGGGGGGAuuuccguuUGCGUc -3' miRNA: 3'- gUGG-GUACua-CGCCCUCUCCU--------ACGCA- -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 132918 | 1.07 | 0.003125 |
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Target: 5'- cCACCCAUGAUGCGGGAGAGGAUGCGUg -3' miRNA: 3'- -GUGGGUACUACGCCCUCUCCUACGCA- -5' |
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| 9902 | 5' | -56.6 | NC_002641.1 | + | 149555 | 1.07 | 0.003125 |
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Target: 5'- cCACCCAUGAUGCGGGAGAGGAUGCGUg -3' miRNA: 3'- -GUGGGUACUACGCCCUCUCCUACGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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