Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9903 | 5' | -56.1 | NC_002641.1 | + | 5436 | 0.67 | 0.871791 |
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Target: 5'- ----cGGGGGCGGGgguaGCGGCGg--- -3' miRNA: 3'- acucuCCUCCGCCCaa--CGCCGUauac -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 118287 | 0.67 | 0.871791 |
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Target: 5'- ----cGGGGGCGGGgguaGCGGCGg--- -3' miRNA: 3'- acucuCCUCCGCCCaa--CGCCGUauac -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 438 | 0.68 | 0.827329 |
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Target: 5'- aUGGGaAGGGGGCcaucgguuuguuuggGGGgaGCGGUGUGUa -3' miRNA: 3'- -ACUC-UCCUCCG---------------CCCaaCGCCGUAUAc -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 123285 | 0.68 | 0.827329 |
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Target: 5'- aUGGGaAGGGGGCcaucgguuuguuuggGGGgaGCGGUGUGUa -3' miRNA: 3'- -ACUC-UCCUCCG---------------CCCaaCGCCGUAUAc -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 125597 | 0.71 | 0.629476 |
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Target: 5'- cGGGAccGGAuGGCGGGggGgGGguUGUGg -3' miRNA: 3'- aCUCU--CCU-CCGCCCaaCgCCguAUAC- -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 156875 | 0.71 | 0.629476 |
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Target: 5'- cGGGAccGGAuGGCGGGggGgGGguUGUGg -3' miRNA: 3'- aCUCU--CCU-CCGCCCaaCgCCguAUAC- -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 149013 | 0.8 | 0.234139 |
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Target: 5'- gGAGAGGAGGCGGG--GC-GCAUAUGc -3' miRNA: 3'- aCUCUCCUCCGCCCaaCGcCGUAUAC- -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 133460 | 0.8 | 0.234139 |
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Target: 5'- gGAGAGGAGGCGGG--GC-GCAUAUGc -3' miRNA: 3'- aCUCUCCUCCGCCCaaCGcCGUAUAC- -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 133661 | 1.08 | 0.003302 |
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Target: 5'- gUGAGAGGAGGCGGGUUGCGGCAUAUGc -3' miRNA: 3'- -ACUCUCCUCCGCCCAACGCCGUAUAC- -5' |
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| 9903 | 5' | -56.1 | NC_002641.1 | + | 148811 | 1.08 | 0.003302 |
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Target: 5'- gUGAGAGGAGGCGGGUUGCGGCAUAUGc -3' miRNA: 3'- -ACUCUCCUCCGCCCAACGCCGUAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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