Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9905 | 5' | -49.4 | NC_002641.1 | + | 77553 | 0.66 | 0.998346 |
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Target: 5'- gGAuGGGGGAGUGuuAGACGuuugcauaauGGAUGUGUu -3' miRNA: 3'- gCU-CUCCUUCAC--UCUGU----------CCUAUACAc -5' |
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| 9905 | 5' | -49.4 | NC_002641.1 | + | 16567 | 0.67 | 0.99607 |
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Target: 5'- -uAGAGGAAGUGuuGGGCAGGGa----- -3' miRNA: 3'- gcUCUCCUUCAC--UCUGUCCUauacac -5' |
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| 9905 | 5' | -49.4 | NC_002641.1 | + | 147152 | 0.76 | 0.776695 |
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Target: 5'- cCGGGAGGAAGUGAGACAGc------- -3' miRNA: 3'- -GCUCUCCUUCACUCUGUCcuauacac -5' |
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| 9905 | 5' | -49.4 | NC_002641.1 | + | 135321 | 0.76 | 0.776695 |
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Target: 5'- cCGGGAGGAAGUGAGACAGc------- -3' miRNA: 3'- -GCUCUCCUUCACUCUGUCcuauacac -5' |
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| 9905 | 5' | -49.4 | NC_002641.1 | + | 135473 | 1.09 | 0.012723 |
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Target: 5'- aCGAGAGGAAGUGAGACAGGAUAUGUGc -3' miRNA: 3'- -GCUCUCCUUCACUCUGUCCUAUACAC- -5' |
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| 9905 | 5' | -49.4 | NC_002641.1 | + | 146999 | 1.09 | 0.012723 |
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Target: 5'- aCGAGAGGAAGUGAGACAGGAUAUGUGc -3' miRNA: 3'- -GCUCUCCUUCACUCUGUCCUAUACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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