Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9907 | 5' | -52.8 | NC_002641.1 | + | 31762 | 0.66 | 0.9753 |
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Target: 5'- gCCGGCGauacaCCGGaaaaaUGUAUGAUGUCCGc- -3' miRNA: 3'- -GGCUGC-----GGUUg----ACGUACUGCAGGUau -5' |
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| 9907 | 5' | -52.8 | NC_002641.1 | + | 64658 | 0.66 | 0.9753 |
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Target: 5'- gCCGcaACGCCAACUGUGaGACGUg---- -3' miRNA: 3'- -GGC--UGCGGUUGACGUaCUGCAgguau -5' |
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| 9907 | 5' | -52.8 | NC_002641.1 | + | 136741 | 0.71 | 0.846541 |
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Target: 5'- aCCGACGgCuGCcGCGUgGACGUCCGc- -3' miRNA: 3'- -GGCUGCgGuUGaCGUA-CUGCAGGUau -5' |
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| 9907 | 5' | -52.8 | NC_002641.1 | + | 145731 | 0.71 | 0.846541 |
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Target: 5'- aCCGACGgCuGCcGCGUgGACGUCCGc- -3' miRNA: 3'- -GGCUGCgGuUGaCGUA-CUGCAGGUau -5' |
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| 9907 | 5' | -52.8 | NC_002641.1 | + | 145885 | 1.09 | 0.006265 |
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Target: 5'- gCCGACGCCAACUGCAUGACGUCCAUAu -3' miRNA: 3'- -GGCUGCGGUUGACGUACUGCAGGUAU- -5' |
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| 9907 | 5' | -52.8 | NC_002641.1 | + | 136588 | 1.09 | 0.006265 |
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Target: 5'- gCCGACGCCAACUGCAUGACGUCCAUAu -3' miRNA: 3'- -GGCUGCGGUUGACGUACUGCAGGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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