Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9909 | 5' | -55.4 | NC_002641.1 | + | 148129 | 1.09 | 0.003557 |
Target: 5'- cCGAUGCCGAUCUCGCACACAGAGGCUg -3' miRNA: 3'- -GCUACGGCUAGAGCGUGUGUCUCCGA- -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 134344 | 1.09 | 0.003557 |
Target: 5'- cCGAUGCCGAUCUCGCACACAGAGGCUg -3' miRNA: 3'- -GCUACGGCUAGAGCGUGUGUCUCCGA- -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 118846 | 0.81 | 0.214586 |
Target: 5'- aGAUGCCGAuuUCUCGCACACcuGGAccGGCUc -3' miRNA: 3'- gCUACGGCU--AGAGCGUGUG--UCU--CCGA- -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 4877 | 0.81 | 0.214586 |
Target: 5'- aGAUGCCGAuuUCUCGCACACcuGGAccGGCUc -3' miRNA: 3'- gCUACGGCU--AGAGCGUGUG--UCU--CCGA- -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 118060 | 0.72 | 0.664127 |
Target: 5'- aGcgGCCGAUCa-GCGCGCGGcGGGCc -3' miRNA: 3'- gCuaCGGCUAGagCGUGUGUC-UCCGa -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 5662 | 0.72 | 0.664127 |
Target: 5'- aGcgGCCGAUCa-GCGCGCGGcGGGCc -3' miRNA: 3'- gCuaCGGCUAGagCGUGUGUC-UCCGa -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 139173 | 0.68 | 0.859291 |
Target: 5'- ---aGCaGAUCUCGCAaccucCAgGGAGGCUa -3' miRNA: 3'- gcuaCGgCUAGAGCGU-----GUgUCUCCGA- -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 140823 | 0.68 | 0.866141 |
Target: 5'- uGAcUGCCGGUCUCGUACuacuuucGCAcAGGUg -3' miRNA: 3'- gCU-ACGGCUAGAGCGUG-------UGUcUCCGa -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 45649 | 0.68 | 0.866892 |
Target: 5'- aGAUGCUGAUaCUgGCA-GCAGAGGa- -3' miRNA: 3'- gCUACGGCUA-GAgCGUgUGUCUCCga -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 117764 | 0.66 | 0.930481 |
Target: 5'- gGGUGgCGcgg-CGCAUACGGGGGCc -3' miRNA: 3'- gCUACgGCuagaGCGUGUGUCUCCGa -5' |
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9909 | 5' | -55.4 | NC_002641.1 | + | 80296 | 0.66 | 0.930481 |
Target: 5'- -cGUGCCG-UUUCGUGCAaagcaugccCGGAGGCa -3' miRNA: 3'- gcUACGGCuAGAGCGUGU---------GUCUCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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