Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9914 | 3' | -57.3 | NC_002641.1 | + | 46351 | 0.66 | 0.852078 |
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Target: 5'- uGACGacAGCGaacguuaucuuuuaUCUGCC-GAUCGGUCAg -3' miRNA: 3'- -CUGCguUCGC--------------AGACGGuCUAGCCGGU- -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 51094 | 0.66 | 0.846536 |
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Target: 5'- uGGCGCAuuauuucugAGCGgagacggCUaGCCGGAauugUUGGCCAg -3' miRNA: 3'- -CUGCGU---------UCGCa------GA-CGGUCU----AGCCGGU- -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 154347 | 0.67 | 0.820884 |
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Target: 5'- aGACGCAgccuccccaGGCGUUUGCUgcgguggaugcggAGA-CGGCCc -3' miRNA: 3'- -CUGCGU---------UCGCAGACGG-------------UCUaGCCGGu -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 128125 | 0.67 | 0.820884 |
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Target: 5'- aGACGCAgccuccccaGGCGUUUGCUgcgguggaugcggAGA-CGGCCc -3' miRNA: 3'- -CUGCGU---------UCGCAGACGG-------------UCUaGCCGGu -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 124720 | 0.67 | 0.807868 |
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Target: 5'- uGACaGCua--GUCUGCCGGAucugcauucccguacUCGGCCGc -3' miRNA: 3'- -CUG-CGuucgCAGACGGUCU---------------AGCCGGU- -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 129168 | 0.67 | 0.795397 |
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Target: 5'- aGACGCuuGCGUCcugGCCugcGAUCGGgUAa -3' miRNA: 3'- -CUGCGuuCGCAGa--CGGu--CUAGCCgGU- -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 153305 | 0.67 | 0.795397 |
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Target: 5'- aGACGCuuGCGUCcugGCCugcGAUCGGgUAa -3' miRNA: 3'- -CUGCGuuCGCAGa--CGGu--CUAGCCgGU- -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 153476 | 1.08 | 0.002325 |
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Target: 5'- gGACGCAAGCGUCUGCCAGAUCGGCCAa -3' miRNA: 3'- -CUGCGUUCGCAGACGGUCUAGCCGGU- -5' |
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| 9914 | 3' | -57.3 | NC_002641.1 | + | 128996 | 1.08 | 0.002325 |
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Target: 5'- gGACGCAAGCGUCUGCCAGAUCGGCCAa -3' miRNA: 3'- -CUGCGUUCGCAGACGGUCUAGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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