Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9919 | 5' | -51.8 | NC_002641.1 | + | 11712 | 0.66 | 0.991003 |
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Target: 5'- -cCUGAGGggcUCCCCCCCGGc------- -3' miRNA: 3'- auGACUUU---AGGGGGGGCCaaacuacc -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 37798 | 0.66 | 0.98504 |
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Target: 5'- -uCUGAcacgcgGAUCCCCCCCGcuUUUGAa-- -3' miRNA: 3'- auGACU------UUAGGGGGGGCc-AAACUacc -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 129903 | 0.66 | 0.98504 |
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Target: 5'- cACUGAuagaaguucccGAcCCCCCCCgcgguuccuguuGGUUcGGUGGg -3' miRNA: 3'- aUGACU-----------UUaGGGGGGG------------CCAAaCUACC- -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 152570 | 0.66 | 0.98504 |
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Target: 5'- cACUGAuagaaguucccGAcCCCCCCCgcgguuccuguuGGUUcGGUGGg -3' miRNA: 3'- aUGACU-----------UUaGGGGGGG------------CCAAaCUACC- -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 148375 | 0.67 | 0.98111 |
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Target: 5'- --------aCCCCCCgCGGUguuaUGAUGGg -3' miRNA: 3'- augacuuuaGGGGGG-GCCAa---ACUACC- -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 134097 | 0.67 | 0.98111 |
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Target: 5'- --------aCCCCCCgCGGUguuaUGAUGGg -3' miRNA: 3'- augacuuuaGGGGGG-GCCAa---ACUACC- -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 122976 | 1.11 | 0.006058 |
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Target: 5'- aUACUGAAAUCCCCCCCGGUUUGAUGGg -3' miRNA: 3'- -AUGACUUUAGGGGGGGCCAAACUACC- -5' |
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| 9919 | 5' | -51.8 | NC_002641.1 | + | 747 | 1.11 | 0.006058 |
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Target: 5'- aUACUGAAAUCCCCCCCGGUUUGAUGGg -3' miRNA: 3'- -AUGACUUUAGGGGGGGCCAAACUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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