Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9926 | 5' | -59.4 | NC_002641.1 | + | 117083 | 0.67 | 0.689933 |
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Target: 5'- cCGCGCCcgGCGCGCggUCCG-GAGGGAg -3' miRNA: 3'- uGUGCGGa-UGCGUG--GGGCgCUCUCUa -5' |
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| 9926 | 5' | -59.4 | NC_002641.1 | + | 86691 | 0.69 | 0.569791 |
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Target: 5'- gGCACGCUaUugGCAUCCCGCGucauGAa -3' miRNA: 3'- -UGUGCGG-AugCGUGGGGCGCucu-CUa -5' |
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| 9926 | 5' | -59.4 | NC_002641.1 | + | 137962 | 0.69 | 0.569791 |
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Target: 5'- aACuCGCCUucgucuauaugGCGgACCCCGCGGGAa-- -3' miRNA: 3'- -UGuGCGGA-----------UGCgUGGGGCGCUCUcua -5' |
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| 9926 | 5' | -59.4 | NC_002641.1 | + | 5717 | 0.7 | 0.540248 |
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Target: 5'- gGCGCGCCgagcGCGCGCUagcugaUCGCGcAGGGAUg -3' miRNA: 3'- -UGUGCGGa---UGCGUGG------GGCGC-UCUCUA- -5' |
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| 9926 | 5' | -59.4 | NC_002641.1 | + | 118005 | 0.7 | 0.540248 |
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Target: 5'- gGCGCGCCgagcGCGCGCUagcugaUCGCGcAGGGAUg -3' miRNA: 3'- -UGUGCGGa---UGCGUGG------GGCGC-UCUCUA- -5' |
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| 9926 | 5' | -59.4 | NC_002641.1 | + | 121300 | 1.04 | 0.002781 |
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Target: 5'- cACACGCCUACGCACCCCGCGAGAGAUc -3' miRNA: 3'- -UGUGCGGAUGCGUGGGGCGCUCUCUA- -5' |
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| 9926 | 5' | -59.4 | NC_002641.1 | + | 2422 | 1.04 | 0.002781 |
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Target: 5'- cACACGCCUACGCACCCCGCGAGAGAUc -3' miRNA: 3'- -UGUGCGGAUGCGUGGGGCGCUCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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