Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9934 | 5' | -53.8 | NC_002641.1 | + | 128717 | 0.66 | 0.954039 |
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Target: 5'- aGggGGGCgGGCGCGCGGaaaCUAc- -3' miRNA: 3'- gCuuCUUGaCCGCGCGUCguaGGUag -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 153755 | 0.66 | 0.954039 |
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Target: 5'- aGggGGGCgGGCGCGCGGaaaCUAc- -3' miRNA: 3'- gCuuCUUGaCCGCGCGUCguaGGUag -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 127491 | 0.66 | 0.949898 |
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Target: 5'- gGAuGggUUGGUGCGCuagaguaucugcAGCAUCUAa- -3' miRNA: 3'- gCUuCuuGACCGCGCG------------UCGUAGGUag -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 154981 | 0.66 | 0.949898 |
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Target: 5'- gGAuGggUUGGUGCGCuagaguaucugcAGCAUCUAa- -3' miRNA: 3'- gCUuCuuGACCGCGCG------------UCGUAGGUag -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 11534 | 0.67 | 0.94089 |
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Target: 5'- aGggGAGCUGGC-UGCAucaacuuauacGCAUCCc-- -3' miRNA: 3'- gCuuCUUGACCGcGCGU-----------CGUAGGuag -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 77764 | 0.67 | 0.930895 |
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Target: 5'- ---cGAACUGGaUG-GCGGCGUCCAacUCg -3' miRNA: 3'- gcuuCUUGACC-GCgCGUCGUAGGU--AG- -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 56248 | 0.68 | 0.901558 |
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Target: 5'- uGGuuGGAACUaGuGCGCGCGGCGUCgGc- -3' miRNA: 3'- gCU--UCUUGA-C-CGCGCGUCGUAGgUag -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 76104 | 0.68 | 0.901558 |
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Target: 5'- -aAAGAGCUGGCGaGUuuAGCG-CCAUCc -3' miRNA: 3'- gcUUCUUGACCGCgCG--UCGUaGGUAG- -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 117073 | 0.7 | 0.804062 |
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Target: 5'- uCGggGucucccggugGGCGUuCAGCGUCCAUCg -3' miRNA: 3'- -GCuuCuuga------CCGCGcGUCGUAGGUAG- -5' |
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| 9934 | 5' | -53.8 | NC_002641.1 | + | 39508 | 1.1 | 0.00346 |
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Target: 5'- uCGAAGAACUGGCGCGCAGCAUCCAUCa -3' miRNA: 3'- -GCUUCUUGACCGCGCGUCGUAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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