Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9939 | 3' | -55.7 | NC_002641.1 | + | 48061 | 0.67 | 0.881346 |
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Target: 5'- --cGCGCaacaGGGACUaagcaGUUGCUGGAGUCu -3' miRNA: 3'- aguCGUGg---CUCUGA-----CGACGGCUUCAG- -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 24783 | 0.69 | 0.753312 |
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Target: 5'- aCAGCGCUGcu-CUGCUGgcuUCGAAGUCu -3' miRNA: 3'- aGUCGUGGCucuGACGAC---GGCUUCAG- -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 153590 | 0.72 | 0.601096 |
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Target: 5'- -aAGCGgCGAGGCggGCUcGUCGggGUCg -3' miRNA: 3'- agUCGUgGCUCUGa-CGA-CGGCuuCAG- -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 154694 | 0.72 | 0.601096 |
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Target: 5'- aUCAGUGCCGcGGCgGCUGCgGGAGg- -3' miRNA: 3'- -AGUCGUGGCuCUGaCGACGgCUUCag -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 127778 | 0.72 | 0.601096 |
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Target: 5'- aUCAGUGCCGcGGCgGCUGCgGGAGg- -3' miRNA: 3'- -AGUCGUGGCuCUGaCGACGgCUUCag -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 128882 | 0.72 | 0.601096 |
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Target: 5'- -aAGCGgCGAGGCggGCUcGUCGggGUCg -3' miRNA: 3'- agUCGUgGCUCUGa-CGA-CGGCuuCAG- -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 4908 | 0.75 | 0.453085 |
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Target: 5'- uUCGGCGCgGuuGGCUGCcGCCGAcGGUCa -3' miRNA: 3'- -AGUCGUGgCu-CUGACGaCGGCU-UCAG- -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 118814 | 0.75 | 0.453085 |
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Target: 5'- uUCGGCGCgGuuGGCUGCcGCCGAcGGUCa -3' miRNA: 3'- -AGUCGUGgCu-CUGACGaCGGCU-UCAG- -5' |
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| 9939 | 3' | -55.7 | NC_002641.1 | + | 75580 | 1.1 | 0.002542 |
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Target: 5'- uUCAGCACCGAGACUGCUGCCGAAGUCu -3' miRNA: 3'- -AGUCGUGGCUCUGACGACGGCUUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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