miRNA: 3'- aUCGCa-CAGCGc-AGCCGCUAGcuCUG- -5'
miRNA: 3'- aUCGCACAGCG--CAGCCGCUAgCUCug -5'
miRNA: 3'- auCGCa-------CAGCGCAgcCGCUAGCUCUG- -5'
miRNA: 3'- -AUCGCACAGCGCAgcCGCUAGCUCUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9942 | 3' | -56.7 | NC_002641.1 | + | 43563 | 0.66 | 0.893032 |
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Target: 5'- gAGCGcgGUCGCcuUCGGCGAUUc-GACu -3' miRNA: 3'- aUCGCa-CAGCGc-AGCCGCUAGcuCUG- -5' |
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| 9942 | 3' | -56.7 | NC_002641.1 | + | 9670 | 0.72 | 0.592941 |
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Target: 5'- cAGCGUGUCGUggGUUGGCGGUaGAGu- -3' miRNA: 3'- aUCGCACAGCG--CAGCCGCUAgCUCug -5' |
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| 9942 | 3' | -56.7 | NC_002641.1 | + | 99255 | 0.75 | 0.430007 |
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Target: 5'- -uGCGgcaucuagGUCGCGUgcGCGAUCGAGACg -3' miRNA: 3'- auCGCa-------CAGCGCAgcCGCUAGCUCUG- -5' |
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| 9942 | 3' | -56.7 | NC_002641.1 | + | 99028 | 0.9 | 0.047026 |
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Target: 5'- cUAGCGUGUCGCGUgcGCGAUCGAGACg -3' miRNA: 3'- -AUCGCACAGCGCAgcCGCUAGCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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