Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9944 | 3' | -45.9 | NC_002641.1 | + | 28500 | 0.67 | 0.999868 |
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Target: 5'- cUCACAUGGUuGCGUCUggccgUGGcCUa- -3' miRNA: 3'- -GGUGUACCGuCGUAGAaa---AUCaGAaa -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 60171 | 0.67 | 0.999776 |
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Target: 5'- uCCGCAuuUGGCAGCAaacccaUUGGUCg-- -3' miRNA: 3'- -GGUGU--ACCGUCGUagaa--AAUCAGaaa -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 67619 | 0.67 | 0.999776 |
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Target: 5'- aCAUaAUGGauaAGCAUUUUUUGGUCg-- -3' miRNA: 3'- gGUG-UACCg--UCGUAGAAAAUCAGaaa -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 72900 | 0.68 | 0.999536 |
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Target: 5'- gCCACGUGGUAGCAUUUUc-------- -3' miRNA: 3'- -GGUGUACCGUCGUAGAAaaucagaaa -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 58468 | 0.68 | 0.99927 |
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Target: 5'- aUACuAUGGCAGgAUCUcUUGGUUUUUu -3' miRNA: 3'- gGUG-UACCGUCgUAGAaAAUCAGAAA- -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 72335 | 0.71 | 0.994339 |
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Target: 5'- aCCACAUGGCAGCuaaugCagUUGGUg--- -3' miRNA: 3'- -GGUGUACCGUCGua---GaaAAUCAgaaa -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 109006 | 0.75 | 0.950095 |
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Target: 5'- -gACAUGGUGGaAUCUUUUAGUCUg- -3' miRNA: 3'- ggUGUACCGUCgUAGAAAAUCAGAaa -5' |
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| 9944 | 3' | -45.9 | NC_002641.1 | + | 108782 | 1.09 | 0.023851 |
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Target: 5'- gCCACAUGGCAGCAUCUUUUAGUCUUUg -3' miRNA: 3'- -GGUGUACCGUCGUAGAAAAUCAGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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