Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 995 | 3' | -42.6 | NC_000902.1 | + | 16630 | 0.66 | 0.999932 |
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Target: 5'- gGCCUCAguuagCCAGug----AGCGCAc-- -3' miRNA: 3'- -CGGAGUa----GGUCuuuuaaUUGCGUuua -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 49651 | 0.67 | 0.999777 |
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Target: 5'- -aCUCAgCCAGAAAGaggGGCGCAc-- -3' miRNA: 3'- cgGAGUaGGUCUUUUaa-UUGCGUuua -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 31410 | 0.67 | 0.999777 |
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Target: 5'- aCCUCAUCCugugccGGAA--UGGCGCGGGa -3' miRNA: 3'- cGGAGUAGGu-----CUUUuaAUUGCGUUUa -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 32093 | 0.69 | 0.998734 |
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Target: 5'- gGCa-CAUCCAGAAGAUUAACccaCAGGUc -3' miRNA: 3'- -CGgaGUAGGUCUUUUAAUUGc--GUUUA- -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 36850 | 0.69 | 0.998427 |
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Target: 5'- aGCCUCuUCCAGuGGAAcUGGCGUAu-- -3' miRNA: 3'- -CGGAGuAGGUC-UUUUaAUUGCGUuua -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 25653 | 0.69 | 0.99806 |
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Target: 5'- uUUUCAUCCAGAAacuggucgagGAUUucaGGCGCAGAa -3' miRNA: 3'- cGGAGUAGGUCUU----------UUAA---UUGCGUUUa -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 52279 | 0.69 | 0.99806 |
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Target: 5'- cGCCUUAUCCuGAAca-UAACGCu--- -3' miRNA: 3'- -CGGAGUAGGuCUUuuaAUUGCGuuua -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 29185 | 0.7 | 0.995795 |
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Target: 5'- cGCCUUAgCCGGAuauUUcGCGCAAAUu -3' miRNA: 3'- -CGGAGUaGGUCUuuuAAuUGCGUUUA- -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 54018 | 0.72 | 0.986601 |
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Target: 5'- -aCUCAUCCAGAucggccuGCGCAAu- -3' miRNA: 3'- cgGAGUAGGUCUuuuaau-UGCGUUua -5' |
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| 995 | 3' | -42.6 | NC_000902.1 | + | 60627 | 1.09 | 0.02239 |
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Target: 5'- uGCCUCAUCCAGAAAAUUAACGCAAAUu -3' miRNA: 3'- -CGGAGUAGGUCUUUUAAUUGCGUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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