miRNA display CGI


Results 1 - 15 of 15 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9957 5' -60.1 NC_002641.1 + 42459 0.66 0.724785
Target:  5'- uGGCAGCauauauucgACGcGAauugucgcGGCGGGGGAUGa -3'
miRNA:   3'- -UCGUCGcca------UGCuCU--------CCGCCCCCUAC- -5'
9957 5' -60.1 NC_002641.1 + 88037 0.67 0.666051
Target:  5'- uGCAGCGG-ACauGAGGacgaGGGGGAa- -3'
miRNA:   3'- uCGUCGCCaUGcuCUCCg---CCCCCUac -5'
9957 5' -60.1 NC_002641.1 + 130704 0.69 0.57667
Target:  5'- uGGaCuGCGGc-CGAGAGGaCGGGGGGg- -3'
miRNA:   3'- -UC-GuCGCCauGCUCUCC-GCCCCCUac -5'
9957 5' -60.1 NC_002641.1 + 151769 0.69 0.57667
Target:  5'- uGGaCuGCGGc-CGAGAGGaCGGGGGGg- -3'
miRNA:   3'- -UC-GuCGCCauGCUCUCC-GCCCCCUac -5'
9957 5' -60.1 NC_002641.1 + 151431 0.69 0.566839
Target:  5'- gAGCGG-GGUuccuACGccAGGCGGGGGAg- -3'
miRNA:   3'- -UCGUCgCCA----UGCucUCCGCCCCCUac -5'
9957 5' -60.1 NC_002641.1 + 131041 0.69 0.566839
Target:  5'- gAGCGG-GGUuccuACGccAGGCGGGGGAg- -3'
miRNA:   3'- -UCGUCgCCA----UGCucUCCGCCCCCUac -5'
9957 5' -60.1 NC_002641.1 + 132291 0.69 0.554123
Target:  5'- cGCGGCauaaauaugggauuGGUA--GGGGGCGGGGGGUu -3'
miRNA:   3'- uCGUCG--------------CCAUgcUCUCCGCCCCCUAc -5'
9957 5' -60.1 NC_002641.1 + 70175 0.69 0.537626
Target:  5'- uGCuuuGCGGUgugcauuaccACGGGGGGUGuGGGGAa- -3'
miRNA:   3'- uCGu--CGCCA----------UGCUCUCCGC-CCCCUac -5'
9957 5' -60.1 NC_002641.1 + 5532 0.7 0.51844
Target:  5'- aGGCucGGCGGUcuugccgacgaAgGGGAGGgGGGcGGAUGg -3'
miRNA:   3'- -UCG--UCGCCA-----------UgCUCUCCgCCC-CCUAC- -5'
9957 5' -60.1 NC_002641.1 + 118190 0.7 0.51844
Target:  5'- aGGCucGGCGGUcuugccgacgaAgGGGAGGgGGGcGGAUGg -3'
miRNA:   3'- -UCG--UCGCCA-----------UgCUCUCCgCCC-CCUAC- -5'
9957 5' -60.1 NC_002641.1 + 83301 0.73 0.362428
Target:  5'- uGGCAGCGGUuucugugaauaggaGGGAuGGCGGGGG-UGu -3'
miRNA:   3'- -UCGUCGCCAug------------CUCU-CCGCCCCCuAC- -5'
9957 5' -60.1 NC_002641.1 + 133564 0.73 0.331556
Target:  5'- cAGCGGCGGgugUGGGGGGgGGGGGc-- -3'
miRNA:   3'- -UCGUCGCCau-GCUCUCCgCCCCCuac -5'
9957 5' -60.1 NC_002641.1 + 148908 0.73 0.331556
Target:  5'- cAGCGGCGGgugUGGGGGGgGGGGGc-- -3'
miRNA:   3'- -UCGUCGCCau-GCUCUCCgCCCCCuac -5'
9957 5' -60.1 NC_002641.1 + 131180 1.08 0.001367
Target:  5'- aAGCAGCGGUACGAGAGGCGGGGGAUGg -3'
miRNA:   3'- -UCGUCGCCAUGCUCUCCGCCCCCUAC- -5'
9957 5' -60.1 NC_002641.1 + 151292 1.08 0.001367
Target:  5'- aAGCAGCGGUACGAGAGGCGGGGGAUGg -3'
miRNA:   3'- -UCGUCGCCAUGCUCUCCGCCCCCUAC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.