Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9958 | 3' | -55.4 | NC_002641.1 | + | 106957 | 0.66 | 0.940974 |
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Target: 5'- aACACGCAUgCCUauuuuaUCCUGaCAgccgCAUGGa -3' miRNA: 3'- -UGUGCGUA-GGAg-----AGGGC-GUa---GUACCc -5' |
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| 9958 | 3' | -55.4 | NC_002641.1 | + | 9972 | 0.66 | 0.931218 |
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Target: 5'- -gGCGguUCCgcCUCCCGagcaaaCAUGGGg -3' miRNA: 3'- ugUGCguAGGa-GAGGGCgua---GUACCC- -5' |
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| 9958 | 3' | -55.4 | NC_002641.1 | + | 91484 | 0.66 | 0.925984 |
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Target: 5'- --uCGaUGUCCUCuUCCCGCAgugCAUuGGGg -3' miRNA: 3'- uguGC-GUAGGAG-AGGGCGUa--GUA-CCC- -5' |
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| 9958 | 3' | -55.4 | NC_002641.1 | + | 86691 | 0.68 | 0.853083 |
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Target: 5'- gGCACGCuAUUggCaUCCCGCGUCAUGa- -3' miRNA: 3'- -UGUGCG-UAGgaG-AGGGCGUAGUACcc -5' |
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| 9958 | 3' | -55.4 | NC_002641.1 | + | 132731 | 0.98 | 0.018354 |
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Target: 5'- aACA-GCAUCCUCUCCCGCAUCAUGGGu -3' miRNA: 3'- -UGUgCGUAGGAGAGGGCGUAGUACCC- -5' |
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| 9958 | 3' | -55.4 | NC_002641.1 | + | 149742 | 1.1 | 0.003213 |
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Target: 5'- aACACGCAUCCUCUCCCGCAUCAUGGGu -3' miRNA: 3'- -UGUGCGUAGGAGAGGGCGUAGUACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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