Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9968 | 3' | -52.9 | NC_002641.1 | + | 12860 | 0.66 | 0.971833 |
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Target: 5'- uGCACaUCCCCuccCCAGAcUGGcuuGUUCa -3' miRNA: 3'- uCGUG-AGGGGuucGGUCUaACC---UAAG- -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 107000 | 0.67 | 0.954549 |
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Target: 5'- uAGCACUCCCUAucAGCagguaAGGUggugGGAc-- -3' miRNA: 3'- -UCGUGAGGGGU--UCGg----UCUAa---CCUaag -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 21053 | 0.67 | 0.936386 |
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Target: 5'- cAGCAUUCUCUA--UCAGAgUGGGUUCg -3' miRNA: 3'- -UCGUGAGGGGUucGGUCUaACCUAAG- -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 115725 | 0.68 | 0.914162 |
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Target: 5'- uGguCUCUCCAAGCCGGuuuucGAUUCu -3' miRNA: 3'- uCguGAGGGGUUCGGUCuaac-CUAAG- -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 156102 | 0.69 | 0.86486 |
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Target: 5'- aGGCGCUCCCgCAaauacacAGCCGGGUcggcgGGAa-- -3' miRNA: 3'- -UCGUGAGGG-GU-------UCGGUCUAa----CCUaag -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 126371 | 0.69 | 0.86486 |
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Target: 5'- aGGCGCUCCCgCAaauacacAGCCGGGUcggcgGGAa-- -3' miRNA: 3'- -UCGUGAGGG-GU-------UCGGUCUAa----CCUaag -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 31186 | 0.71 | 0.778016 |
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Target: 5'- uGUACUCCUCGAGCCAc-UUGGAa-- -3' miRNA: 3'- uCGUGAGGGGUUCGGUcuAACCUaag -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 130568 | 1.1 | 0.004354 |
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Target: 5'- cAGCACUCCCCAAGCCAGAUUGGAUUCu -3' miRNA: 3'- -UCGUGAGGGGUUCGGUCUAACCUAAG- -5' |
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| 9968 | 3' | -52.9 | NC_002641.1 | + | 151904 | 1.1 | 0.004354 |
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Target: 5'- cAGCACUCCCCAAGCCAGAUUGGAUUCu -3' miRNA: 3'- -UCGUGAGGGGUUCGGUCUAACCUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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