Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9968 | 5' | -58.3 | NC_002641.1 | + | 10312 | 0.66 | 0.817845 |
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Target: 5'- cGCGACACCCgaUCCCc---GGGAugUCg -3' miRNA: 3'- -CGCUGUGGGg-AGGGccgaCUCUugAG- -5' |
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| 9968 | 5' | -58.3 | NC_002641.1 | + | 153611 | 0.67 | 0.80062 |
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Target: 5'- cGCGGCACUuuUCCCacuggcaagcGGCgagGcGGGCUCg -3' miRNA: 3'- -CGCUGUGGggAGGG----------CCGa--CuCUUGAG- -5' |
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| 9968 | 5' | -58.3 | NC_002641.1 | + | 128861 | 0.67 | 0.80062 |
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Target: 5'- cGCGGCACUuuUCCCacuggcaagcGGCgagGcGGGCUCg -3' miRNA: 3'- -CGCUGUGGggAGGG----------CCGa--CuCUUGAG- -5' |
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| 9968 | 5' | -58.3 | NC_002641.1 | + | 37761 | 0.7 | 0.607341 |
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Target: 5'- --uGCACCCCU-CCGGCaUGAGGcCUCu -3' miRNA: 3'- cgcUGUGGGGAgGGCCG-ACUCUuGAG- -5' |
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| 9968 | 5' | -58.3 | NC_002641.1 | + | 130534 | 1.1 | 0.001674 |
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Target: 5'- uGCGACACCCCUCCCGGCUGAGAACUCc -3' miRNA: 3'- -CGCUGUGGGGAGGGCCGACUCUUGAG- -5' |
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| 9968 | 5' | -58.3 | NC_002641.1 | + | 151938 | 1.1 | 0.001674 |
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Target: 5'- uGCGACACCCCUCCCGGCUGAGAACUCc -3' miRNA: 3'- -CGCUGUGGGGAGGGCCGACUCUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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