Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9984 | 3' | -55.6 | NC_002654.1 | + | 65904 | 0.66 | 0.897059 |
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Target: 5'- aGCGUcUUGCACguUGGGCGgaaUGUUaGCUCGu -3' miRNA: 3'- -UGCAaAGCGUG--GCCCGU---ACAG-CGAGC- -5' |
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| 9984 | 3' | -55.6 | NC_002654.1 | + | 20423 | 0.66 | 0.883471 |
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Target: 5'- aACGUuuggugguuacUUCGCGCUGGGCGgcgGaUCGUUUu -3' miRNA: 3'- -UGCA-----------AAGCGUGGCCCGUa--C-AGCGAGc -5' |
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| 9984 | 3' | -55.6 | NC_002654.1 | + | 82297 | 0.67 | 0.876335 |
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Target: 5'- gGCGgaggUCGCAggcguUCGGGCGgugGUCGCa-- -3' miRNA: 3'- -UGCaa--AGCGU-----GGCCCGUa--CAGCGagc -5' |
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| 9984 | 3' | -55.6 | NC_002654.1 | + | 29376 | 0.69 | 0.764881 |
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Target: 5'- aGCGaUUUCGUuguGCCGGGCGUGuucgaucugauccUCGC-CGa -3' miRNA: 3'- -UGC-AAAGCG---UGGCCCGUAC-------------AGCGaGC- -5' |
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| 9984 | 3' | -55.6 | NC_002654.1 | + | 51183 | 1.08 | 0.003492 |
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Target: 5'- aACGUUUCGCACCGGGCAUGUCGCUCGc -3' miRNA: 3'- -UGCAAAGCGUGGCCCGUACAGCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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