miRNA: 3'- uCACUGGUAGUugcUGCGGUGGUUGU- -5'
miRNA: 3'- -UCaCUgGUAGUUGCUGCGGUGGUUGU- -5'
miRNA: 3'- ucaCUGGUAGUUGcUGCGGUGGUUGU- -5'
miRNA: 3'- -UCACUGGUAGUUGCUGCGGUGGUUGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9987 | 3' | -52.1 | NC_002654.1 | + | 121045 | 0.74 | 0.690114 |
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Target: 5'- cGUGGCUGUCGugaAUGCCGCCGACAa -3' miRNA: 3'- uCACUGGUAGUugcUGCGGUGGUUGU- -5' |
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| 9987 | 3' | -52.1 | NC_002654.1 | + | 30938 | 0.76 | 0.542392 |
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Target: 5'- gAGcGAgUGUCGACGAUGUCGCCAACAu -3' miRNA: 3'- -UCaCUgGUAGUUGCUGCGGUGGUUGU- -5' |
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| 9987 | 3' | -52.1 | NC_002654.1 | + | 57814 | 0.86 | 0.159677 |
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Target: 5'- --aGACCGUCGGCcGCGCCGCCAACAa -3' miRNA: 3'- ucaCUGGUAGUUGcUGCGGUGGUUGU- -5' |
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| 9987 | 3' | -52.1 | NC_002654.1 | + | 89011 | 1.09 | 0.005411 |
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Target: 5'- aAGUGACCAUCAACGACGCCACCAACAg -3' miRNA: 3'- -UCACUGGUAGUUGCUGCGGUGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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