Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9990 | 5' | -50.7 | NC_002654.1 | + | 20849 | 0.66 | 0.98981 |
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Target: 5'- cGAAUAUcuuGUUGUgaaCGUGCAUGUUGGGCc -3' miRNA: 3'- -CUUGUG---UAGCAg--GCGCGUGCAACUUG- -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 17033 | 0.66 | 0.986788 |
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Target: 5'- aGugACAUCGcCCGCGCuCGUgguccaaGAAUg -3' miRNA: 3'- cUugUGUAGCaGGCGCGuGCAa------CUUG- -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 40440 | 0.66 | 0.986788 |
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Target: 5'- aAACuuuuCAUCGUCUGCGaCACGUUu--- -3' miRNA: 3'- cUUGu---GUAGCAGGCGC-GUGCAAcuug -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 82152 | 0.67 | 0.978729 |
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Target: 5'- aGGACACAaucUCGUCaccuGCGCGCGUc---- -3' miRNA: 3'- -CUUGUGU---AGCAGg---CGCGUGCAacuug -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 98439 | 0.68 | 0.970635 |
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Target: 5'- cAACACGaagaCGaUCCGCuagaagaacacGCACGUUGGGCa -3' miRNA: 3'- cUUGUGUa---GC-AGGCG-----------CGUGCAACUUG- -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 127410 | 0.68 | 0.960542 |
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Target: 5'- cGAACGCuaagCGcuacUCUGCGCACGUuugugcacacauUGGGCa -3' miRNA: 3'- -CUUGUGua--GC----AGGCGCGUGCA------------ACUUG- -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 14113 | 0.7 | 0.92834 |
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Target: 5'- cGGGCACGUUGUCCauGCGuCGCaaugUGGACa -3' miRNA: 3'- -CUUGUGUAGCAGG--CGC-GUGca--ACUUG- -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 65897 | 0.76 | 0.624036 |
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Target: 5'- uGGACACAgCGUCU-UGCACGUUGGGCg -3' miRNA: 3'- -CUUGUGUaGCAGGcGCGUGCAACUUG- -5' |
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| 9990 | 5' | -50.7 | NC_002654.1 | + | 79857 | 1.11 | 0.006112 |
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Target: 5'- uGAACACAUCGUCCGCGCACGUUGAACg -3' miRNA: 3'- -CUUGUGUAGCAGGCGCGUGCAACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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