Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9992 | 5' | -60.7 | NC_002654.1 | + | 61187 | 0.67 | 0.670734 |
Target: 5'- uCGAAgGuaucauaUCUACGGggacggGCCGCCGCUGUa -3' miRNA: 3'- -GCUUgC-------AGAUGCCgg----CGGCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 82188 | 0.67 | 0.632297 |
Target: 5'- aGAACuUCUucguCGucuuCCGCCGCCGCCa- -3' miRNA: 3'- gCUUGcAGAu---GCc---GGCGGCGGCGGcg -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 117561 | 0.67 | 0.632297 |
Target: 5'- aGAAUGUacgaUACGGCUaauGuuGUCGCCGa -3' miRNA: 3'- gCUUGCAg---AUGCCGG---CggCGGCGGCg -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 82471 | 0.67 | 0.632297 |
Target: 5'- -cGACGcUCagGCGGCgGCgGCgGCCGUa -3' miRNA: 3'- gcUUGC-AGa-UGCCGgCGgCGgCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 107662 | 0.68 | 0.582088 |
Target: 5'- uGAGCGcCggaauCGGUCGCagaccaaUGCCGCUGCu -3' miRNA: 3'- gCUUGCaGau---GCCGGCG-------GCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 16785 | 0.69 | 0.51378 |
Target: 5'- uCGGGCGUUUGuauuucauuuuCGGCaccgucgcgcaCCGCCGCCGCc -3' miRNA: 3'- -GCUUGCAGAU-----------GCCGgc---------GGCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 59234 | 0.7 | 0.460513 |
Target: 5'- -cGACGcaaccaUCUGC-GCCGCCaCCGCCGCc -3' miRNA: 3'- gcUUGC------AGAUGcCGGCGGcGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 117298 | 0.71 | 0.392183 |
Target: 5'- -cAACGUugCUGCaguaGCCGCUGCCGCCGa -3' miRNA: 3'- gcUUGCA--GAUGc---CGGCGGCGGCGGCg -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 126806 | 0.72 | 0.368313 |
Target: 5'- aCGAAUGUa-ACGGCacauauuGUCGUCGCCGCa -3' miRNA: 3'- -GCUUGCAgaUGCCGg------CGGCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 51789 | 0.72 | 0.368313 |
Target: 5'- gGAGCGUCcGCGGCCaugucguacuuGCCaUCGUCGCa -3' miRNA: 3'- gCUUGCAGaUGCCGG-----------CGGcGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 82124 | 0.74 | 0.294189 |
Target: 5'- uCGAACGUCUGCGaccaCCGcCCGaacgccugcgaccuCCGCCGCc -3' miRNA: 3'- -GCUUGCAGAUGCc---GGC-GGC--------------GGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 82332 | 0.74 | 0.270553 |
Target: 5'- uGAGCGUCgccuuccaccACcGCUGCCGCUGCUGCu -3' miRNA: 3'- gCUUGCAGa---------UGcCGGCGGCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 26423 | 0.75 | 0.241072 |
Target: 5'- gGGAUGUauuuguaACGGCUGCUGCUGCUGCg -3' miRNA: 3'- gCUUGCAga-----UGCCGGCGGCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 26331 | 0.77 | 0.194788 |
Target: 5'- --uGCGUaauuuGCCGCCGCCGCCGCa -3' miRNA: 3'- gcuUGCAgaugcCGGCGGCGGCGGCG- -5' |
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9992 | 5' | -60.7 | NC_002654.1 | + | 82304 | 1.1 | 0.000931 |
Target: 5'- cCGAACGUCUACGGCCGCCGCCGCCGCc -3' miRNA: 3'- -GCUUGCAGAUGCCGGCGGCGGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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