Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9994 | 3' | -57.7 | NC_002654.1 | + | 35854 | 0.66 | 0.809081 |
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Target: 5'- cGCGGUCGAauACGU--ACGCAGCCu -3' miRNA: 3'- uUGUCAGCUccUGCGucUGCGUCGGc -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 117980 | 0.66 | 0.781873 |
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Target: 5'- cAGCAGUCGuuGugGUGGGCGCA-UCGu -3' miRNA: 3'- -UUGUCAGCucCugCGUCUGCGUcGGC- -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 16602 | 0.66 | 0.781873 |
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Target: 5'- gGACAGggcggCGGcGGuGCGC-GACGguGCCGa -3' miRNA: 3'- -UUGUCa----GCU-CC-UGCGuCUGCguCGGC- -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 50268 | 0.68 | 0.693927 |
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Target: 5'- -----aCGAGuGGCgaGCGGACGCAGCCa -3' miRNA: 3'- uugucaGCUC-CUG--CGUCUGCGUCGGc -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 82415 | 0.68 | 0.65308 |
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Target: 5'- cGGCGGUCGucGACGCAGuuccaGCGguGgCGg -3' miRNA: 3'- -UUGUCAGCucCUGCGUC-----UGCguCgGC- -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 114112 | 0.7 | 0.560951 |
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Target: 5'- cAGCcGUaacCGAaGACGCAGuCGCAGCCGa -3' miRNA: 3'- -UUGuCA---GCUcCUGCGUCuGCGUCGGC- -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 114175 | 0.98 | 0.008841 |
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Target: 5'- cAACAGUCGcGGACGCAGACGCAGCCGa -3' miRNA: 3'- -UUGUCAGCuCCUGCGUCUGCGUCGGC- -5' |
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| 9994 | 3' | -57.7 | NC_002654.1 | + | 114145 | 1.06 | 0.002592 |
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Target: 5'- cAACAGUCGAGGACGCAGACGCAGCCGu -3' miRNA: 3'- -UUGUCAGCUCCUGCGUCUGCGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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