Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9994 | 5' | -60.1 | NC_002654.1 | + | 35844 | 0.67 | 0.666901 |
Target: 5'- uCGCcagaGGCGCGGucgaauacguACGCAGCCuuGGCCa -3' miRNA: 3'- -GCGc---CUGCGUC----------UGCGUCGGcuUCGGc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 65721 | 0.67 | 0.656932 |
Target: 5'- cCGUGGACGCAGACaaugGC-GCUaaaAAGCCu -3' miRNA: 3'- -GCGCCUGCGUCUG----CGuCGGc--UUCGGc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 82486 | 0.67 | 0.653937 |
Target: 5'- aGCGGugGUggaaggcgacgcucAGGCgGCGGCgGcGGCCGu -3' miRNA: 3'- gCGCCugCG--------------UCUG-CGUCGgCuUCGGC- -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 82276 | 0.67 | 0.626942 |
Target: 5'- gGCGGugguCGCAGACGUucgAGCgGuGGUCGc -3' miRNA: 3'- gCGCCu---GCGUCUGCG---UCGgCuUCGGC- -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 129276 | 0.68 | 0.571194 |
Target: 5'- -aUGGACGCGGACGauGCCGAagaauugcacgcuuuAGCCa -3' miRNA: 3'- gcGCCUGCGUCUGCguCGGCU---------------UCGGc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 16614 | 0.68 | 0.564293 |
Target: 5'- gGCGGuGCGC-GACGguGCCGAAaaugaaauacaaacGCCc -3' miRNA: 3'- gCGCC-UGCGuCUGCguCGGCUU--------------CGGc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 50271 | 0.71 | 0.409961 |
Target: 5'- aGUGGcgaGCGGACGCAGCCaacauGGCCc -3' miRNA: 3'- gCGCCug-CGUCUGCGUCGGcu---UCGGc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 82318 | 0.76 | 0.2132 |
Target: 5'- gGCGGACGUAGAagaucagGCGGCgGAGGUCGc -3' miRNA: 3'- gCGCCUGCGUCUg------CGUCGgCUUCGGC- -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 114121 | 0.79 | 0.129945 |
Target: 5'- cCGaaGACGCAGuCGCAGCCGAAGCa- -3' miRNA: 3'- -GCgcCUGCGUCuGCGUCGGCUUCGgc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 114151 | 0.83 | 0.079681 |
Target: 5'- uCGaGGACGCAGACGCAGCCGuAGCa- -3' miRNA: 3'- -GCgCCUGCGUCUGCGUCGGCuUCGgc -5' |
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9994 | 5' | -60.1 | NC_002654.1 | + | 114181 | 1.08 | 0.001367 |
Target: 5'- uCGCGGACGCAGACGCAGCCGAAGCCGu -3' miRNA: 3'- -GCGCCUGCGUCUGCGUCGGCUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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