miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9995 3' -50.6 NC_002654.1 + 77271 0.66 0.990759
Target:  5'- -cGGUgacgacgACAUUgcUAUCGAUGGCAG-CGAc -3'
miRNA:   3'- uuCCA-------UGUAA--AUGGCUGCCGUCuGCU- -5'
9995 3' -50.6 NC_002654.1 + 112739 0.67 0.98055
Target:  5'- --cGUACGUUUGCUGGCGcCAGugGu -3'
miRNA:   3'- uucCAUGUAAAUGGCUGCcGUCugCu -5'
9995 3' -50.6 NC_002654.1 + 87881 0.75 0.672572
Target:  5'- aAAGGagACGUUuccagUGCCGGCGGCGGAUGu -3'
miRNA:   3'- -UUCCa-UGUAA-----AUGGCUGCCGUCUGCu -5'
9995 3' -50.6 NC_002654.1 + 121016 1.07 0.010486
Target:  5'- cAAGGUACAUUUACCGACGGCAGACGAc -3'
miRNA:   3'- -UUCCAUGUAAAUGGCUGCCGUCUGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.