Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9995 | 5' | -48.7 | NC_002654.1 | + | 74596 | 0.66 | 0.998177 |
Target: 5'- uUGUCGUGc-UGCCGCCcaACGuGAAUUg -3' miRNA: 3'- -ACAGCACuuACGGCGGc-UGUuUUUGA- -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 83919 | 0.66 | 0.997379 |
Target: 5'- cGUCGcGAAgauUGuuGUCGACGuuGACg -3' miRNA: 3'- aCAGCaCUU---ACggCGGCUGUuuUUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 86289 | 0.66 | 0.997379 |
Target: 5'- -aUCGUcGAA--CCGCCGACGAuuGCa -3' miRNA: 3'- acAGCA-CUUacGGCGGCUGUUuuUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 124433 | 0.67 | 0.993099 |
Target: 5'- ---aGUG-AUGaCCGCCGACAAuuaGAACa -3' miRNA: 3'- acagCACuUAC-GGCGGCUGUU---UUUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 58960 | 0.67 | 0.992996 |
Target: 5'- cGUCauuauuaGUGAgucGUGUCGgCGGCGAAGACg -3' miRNA: 3'- aCAG-------CACU---UACGGCgGCUGUUUUUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 125952 | 0.69 | 0.982471 |
Target: 5'- uUGUUGUGGAcaUGCCGCUGAauuGuuACg -3' miRNA: 3'- -ACAGCACUU--ACGGCGGCUgu-UuuUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 124306 | 0.69 | 0.982471 |
Target: 5'- -aUCGUG-AUGUCGCCGAUcuGGAUa -3' miRNA: 3'- acAGCACuUACGGCGGCUGuuUUUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 87323 | 0.72 | 0.925189 |
Target: 5'- gGUCGUGuacGUGCCaaCCGACGAcGACa -3' miRNA: 3'- aCAGCACu--UACGGc-GGCUGUUuUUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 17593 | 0.73 | 0.878409 |
Target: 5'- aGUUGUaaacugacGAcUGCCGCUGAUAAGAACUu -3' miRNA: 3'- aCAGCA--------CUuACGGCGGCUGUUUUUGA- -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 77412 | 0.74 | 0.870694 |
Target: 5'- cGUCGUcAccGCCGCUGACAAuuauGACUa -3' miRNA: 3'- aCAGCAcUuaCGGCGGCUGUUu---UUGA- -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 71964 | 0.75 | 0.819565 |
Target: 5'- aGUCGUGuaguUGCCGUCGGCAcgguAACa -3' miRNA: 3'- aCAGCACuu--ACGGCGGCUGUuu--UUGa -5' |
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9995 | 5' | -48.7 | NC_002654.1 | + | 121050 | 1.08 | 0.013988 |
Target: 5'- cUGUCGUGAAUGCCGCCGACAAAAACUc -3' miRNA: 3'- -ACAGCACUUACGGCGGCUGUUUUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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