Results 41 - 45 of 45 are showing below:
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Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | + | 102178 | 0.66 | 0.999034 |
Target: 5'- gCGagCACCUcGGugAGACCGCg -3' miRNA: 3'- aGUagGUGGA-CUugUCUGGCGa -5' |
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MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | - | 145786 | 0.66 | 0.999217 |
Target: 5'- uUCGUCUugCgGGACAGGUCGCg -3' miRNA: 3'- -AGUAGGugGaCUUGUCUGGCGa -5' |
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MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | - | 29292 | 0.66 | 0.999217 |
Target: 5'- uUUGUCCACCgacGCGcGACCGCg -3' miRNA: 3'- -AGUAGGUGGacuUGU-CUGGCGa -5' |
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MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | + | 112585 | 0.66 | 0.999217 |
Target: 5'- uUCuUCUACCUGGcuggcGCAGugCGCc -3' miRNA: 3'- -AGuAGGUGGACU-----UGUCugGCGa -5' |
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MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | - | 101294 | 0.66 | 0.999217 |
Target: 5'- aUCGUCCGCC-----GGACCGCa -3' miRNA: 3'- -AGUAGGUGGacuugUCUGGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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