miRNA: 3'- aCAGUGUAagACAC------UGGCGCU- -5'
miRNA: 3'- aCAGUGuaAGaCACuGGCGcu -5'
miRNA: 3'- -ACAGUGuaa----GACACUG--GCGCU- -5'
miRNA: 3'- -ACAGuGUAAGACac--UGGCGCU- -5'
miRNA: 3'- -ACAGUGUAAGa-------CACUGGcGCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13460 | 3' | -42.2 | NC_003409.1 | + | 50390 | 0.66 | 1 |
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Target: 5'- aGUUACGUg-UGUGcggaccACCGCGAa -3' miRNA: 3'- aCAGUGUAagACAC------UGGCGCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 52674 | 0.66 | 1 |
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Target: 5'- aGUCACcgUCcGUG-CCGCc- -3' miRNA: 3'- aCAGUGuaAGaCACuGGCGcu -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 90333 | 0.66 | 1 |
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Target: 5'- gUGUCGCcuccgggCUGUGAUguUGCGAu -3' miRNA: 3'- -ACAGUGuaa----GACACUG--GCGCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 131466 | 0.66 | 1 |
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Target: 5'- cUGUCuCGUUCUGcaaaACCGUGAc -3' miRNA: 3'- -ACAGuGUAAGACac--UGGCGCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 29327 | 0.66 | 1 |
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Target: 5'- uUGUCACAUUUagggcaaaGUGGCC-CGAu -3' miRNA: 3'- -ACAGUGUAAGa-------CACUGGcGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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