Results 41 - 48 of 48 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | + | 64905 | 0.66 | 0.962068 |
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Target: 5'- gGCUaCAUGuGUCCGGGCAUcuUCg -3' miRNA: 3'- gCGG-GUGC-CAGGCCCGUGuuAG- -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | + | 64248 | 0.67 | 0.945935 |
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Target: 5'- gGCCCGCGGgcaCGGGCGu---- -3' miRNA: 3'- gCGGGUGCCag-GCCCGUguuag -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | - | 61990 | 0.66 | 0.968727 |
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Target: 5'- gCGCUCGCGGUCacguccUGGGCAUccaccgcgccGAUCu -3' miRNA: 3'- -GCGGGUGCCAG------GCCCGUG----------UUAG- -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | + | 58178 | 0.68 | 0.908113 |
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Target: 5'- -aCUCACGGUUgggCGGGCACAAUg -3' miRNA: 3'- gcGGGUGCCAG---GCCCGUGUUAg -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | - | 50159 | 0.67 | 0.943177 |
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Target: 5'- gCGCCCugGGgCCcuucgugggcaagauGGGCACcGUCu -3' miRNA: 3'- -GCGGGugCCaGG---------------CCCGUGuUAG- -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | + | 43345 | 0.67 | 0.933333 |
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Target: 5'- gCGCCCGCGGUCUGcuauucguccacgauGGaCACcAUCa -3' miRNA: 3'- -GCGGGUGCCAGGC---------------CC-GUGuUAG- -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | - | 41666 | 0.68 | 0.901702 |
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Target: 5'- uCGCCCugGGcUCCGacGGCGCcGUUg -3' miRNA: 3'- -GCGGGugCC-AGGC--CCGUGuUAG- -5' |
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| MIMAT0001584 | hcmv-miR-US33 | -53.5 | NC_006273.1 | - | 35985 | 0.69 | 0.880999 |
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Target: 5'- gCGCCCGagaucUGGgucUCCGGGCACGggCa -3' miRNA: 3'- -GCGGGU-----GCC---AGGCCCGUGUuaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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