miRNA: 3'- -UGUUGAUCGCGuGGUUUGacag -5'
miRNA: 3'- -UGUUGau-CGCGUGGUUUGaCaG- -5'
miRNA: 3'- -UGUUGAUCG--------CGUGGUuUGACAG- -5'
miRNA: 3'- -UGuUGAUCGCGUGGUUUGA-CAG- -5'
miRNA: 3'- -UGUUGAUCGCGUGGUUUGACAG- -5'
Results 21 - 25 of 25 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003421 | ebv-miR-BART11-5p | -44.6 | NC_007605.1 | - | 90191 | 0.7 | 0.997764 |
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Target: 5'- gGCGGCUAGCGUcCCAAACa--- -3' miRNA: 3'- -UGUUGAUCGCGuGGUUUGacag -5' |
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| MIMAT0003421 | ebv-miR-BART11-5p | -44.6 | NC_007605.1 | - | 63245 | 0.7 | 0.997296 |
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Target: 5'- aGCAGCccuGCGCGCCAGACgGcCg -3' miRNA: 3'- -UGUUGau-CGCGUGGUUUGaCaG- -5' |
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| MIMAT0003421 | ebv-miR-BART11-5p | -44.6 | NC_007605.1 | + | 4340 | 0.71 | 0.991241 |
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Target: 5'- aGCAGCUGGUcaagagcgGCAUCGuGCUGUCa -3' miRNA: 3'- -UGUUGAUCG--------CGUGGUuUGACAG- -5' |
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| MIMAT0003421 | ebv-miR-BART11-5p | -44.6 | NC_007605.1 | + | 61471 | 0.78 | 0.840002 |
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Target: 5'- gGCcGCUGGUGCGCCGGGCUcGUCu -3' miRNA: 3'- -UGuUGAUCGCGUGGUUUGA-CAG- -5' |
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| MIMAT0003421 | ebv-miR-BART11-5p | -44.6 | NC_007605.1 | - | 147586 | 1.03 | 0.054754 |
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Target: 5'- cACAACUAGCGCACCAAACUGUCu -3' miRNA: 3'- -UGUUGAUCGCGUGGUUUGACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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