Results 21 - 28 of 28 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 105678 | 0.74 | 0.999426 |
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Target: 5'- gGACGCCGUggGCAAUUACcGGGa -3' miRNA: 3'- -UUGUGGCG--UGUUAAUGaUCCa -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 128989 | 0.66 | 1 |
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Target: 5'- gGACGgCGCACGAcUACgAGGg -3' miRNA: 3'- -UUGUgGCGUGUUaAUGaUCCa -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 129517 | 0.66 | 1 |
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Target: 5'- cGCGCUGCGCGAggACgagcucAGGUu -3' miRNA: 3'- uUGUGGCGUGUUaaUGa-----UCCA- -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 131255 | 0.66 | 1 |
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Target: 5'- --gGCCGCACAGUgggccgGCgGGGg -3' miRNA: 3'- uugUGGCGUGUUAa-----UGaUCCa -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 133350 | 1 | 0.208729 |
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Target: 5'- cAACACCGCACAAUUACUAGGUc -3' miRNA: 3'- -UUGUGGCGUGUUAAUGAUCCA- -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 153322 | 0.66 | 1 |
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Target: 5'- cACACUGCGCAAgaGCaugAGGg -3' miRNA: 3'- uUGUGGCGUGUUaaUGa--UCCa -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 153527 | 0.66 | 1 |
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Target: 5'- uGCGCCGCcagauCAAUcggaUGCUGGGc -3' miRNA: 3'- uUGUGGCGu----GUUA----AUGAUCCa -5' |
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| MIMAT0003430 | rlcv-miR-rL1-4-5p | -40.3 | NC_006146.1 | - | 170697 | 0.66 | 1 |
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Target: 5'- -cCGCCGCGC----GCUGGGg -3' miRNA: 3'- uuGUGGCGUGuuaaUGAUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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