miRNA: 3'- uagUUCGCG---UGGUCUGGCA---GGCU- -5'
miRNA: 3'- uaGUUCGCGUGGUCUGGCa-------GGCU- -5'
miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5'
miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5'
miRNA: 3'- -UAGUUCGCGUGguC----UGGCAGGcu -5'
Results 21 - 25 of 25 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 117485 | 0.68 | 0.90789 |
|
Target: 5'- --aGGGCGCggcACCGGGCCGUgcaCCGAc -3' miRNA: 3'- uagUUCGCG---UGGUCUGGCA---GGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 138010 | 0.76 | 0.470476 |
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Target: 5'- -cCAGGUGCACCGGACCGgcggacccCCGAa -3' miRNA: 3'- uaGUUCGCGUGGUCUGGCa-------GGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 142891 | 1.01 | 0.013856 |
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Target: 5'- cAUCAAGCGCACCAGACCGUCCGAc -3' miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 143083 | 1.01 | 0.013856 |
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Target: 5'- cAUCAAGCGCACCAGACCGUCCGAc -3' miRNA: 3'- -UAGUUCGCGUGGUCUGGCAGGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 150314 | 0.66 | 0.946564 |
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Target: 5'- cAUCAAGUGCACguGcaacGCCGUCUa- -3' miRNA: 3'- -UAGUUCGCGUGguC----UGGCAGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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