miRNA: 3'- uaGUUCGCG---UGGUCUGGCAgGCU- -5'
miRNA: 3'- -UAGUUCGCGUGguC----UGGCAGGcu -5'
miRNA: 3'- uaGUUCGCGUGGUC-----UGGCa----GGCU- -5'
miRNA: 3'- uagUUCGCGUGG-------UCUGGCa-----GGCU- -5'
miRNA: 3'- uagUUCGCGUgGUCuGGCAGGCU- -5'
Results 21 - 25 of 25 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 96536 | 0.67 | 0.941835 |
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Target: 5'- -aCAGGCGCugcACCGGGCUGU-CGAa -3' miRNA: 3'- uaGUUCGCG---UGGUCUGGCAgGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 150314 | 0.66 | 0.946564 |
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Target: 5'- cAUCAAGUGCACguGcaacGCCGUCUa- -3' miRNA: 3'- -UAGUUCGCGUGguC----UGGCAGGcu -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | + | 57985 | 0.66 | 0.951033 |
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Target: 5'- -cCAGGCGCACCAGgacgaGCUGgaggcCCGGg -3' miRNA: 3'- uaGUUCGCGUGGUC-----UGGCa----GGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | - | 94713 | 0.66 | 0.958819 |
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Target: 5'- --aGGGCGCGCCgggggagGGACCGggggcgCCGAc -3' miRNA: 3'- uagUUCGCGUGG-------UCUGGCa-----GGCU- -5' |
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| MIMAT0003444 | rlcv-miR-rL1-14-5p | -52.6 | NC_006146.1 | + | 56796 | 0.66 | 0.962915 |
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Target: 5'- --uGAGCGCGgCGG-CCGUCUGGc -3' miRNA: 3'- uagUUCGCGUgGUCuGGCAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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